565784-M

γ-Secretase Inhibitor IX

≥95% (HPLC), liquid, γ-secretase inhibitor, Calbiochem^®

• Synonym(s): InSolution γ-Secretase Inhibitor IX, N-[N-(3,5-Difluorophenacetyl-L-alanyl)]-S-phenylglycine t-Butyl Ester, DAPT
• Empirical Formula (Hill Notation): C₂₃H₂₆F₂N₂O₄
• Molecular Weight: 432.46
• UNSPSC Code: 12352200

Properties

• product name: γ-Secretase Inhibitor IX, InSolution, ≥95%, 25 mM, g-secretase Inhibitor IX
• Quality Level: 100
• assay: ≥95% (HPLC)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• color: clear colorless
• storage temp.: 2-8°C

Description

General description

A cell-permeable dipeptide that inhibits γ-secretase activity and suppresses Aβ production (Aβ total IC₅₀ = 115 nM; Aβ₄₂ IC₅₀ = 200 nM). Reported to be functionally active in both HEK293 cells overexpressing human APP751 and neuronal cultures, however, it does not affect the secretion of amyloid-b precursor protein (APP). Effective in acutely lowering Ab in APPV717F-transgenic mice. Also effective in inhibiting the production of a ~6 kDa C-terminal cleavage product (CTFg) in HEK293 cells stably transfected with the Swedish mutant, βAPP₆₉₅. Reported to reduce extracellular Aβ plaques and intracellular Aβ accumulation in 3xTgAD transgenic mice.

Biochem/physiol Actions

Primary Target
Aβ_total

Target IC₅₀: 115 nM, 200 nM against Aβ_total, Aβ42, respectively

Warning

Toxicity: Irritant (B)

Physical form

A 25 mM (5 mg/462 µl or 10 mg/924 µl) solution of γ-Secretase Inhibitor IX (Cat. No. 565770) in DMSO.

Reconstitution

Following initial use, aliquot and freeze (-20°C).

Other Notes

Oddo, S., et al. 2004. Neuron43, 321.
Dovey, H.F., et al. 2001. J. Neurochem.76, 173.
Vandermeeren, M., et al. 2001. Neurosci. Lett.315, 145.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: 188.6 °F - closed cup - (refers to pure substance)
• flash_point_c: 87 °C - closed cup - (refers to pure substance)