605160

Thrombin, Plasminogen-Free, Bovine

• Synonym(s): Thrombin, Plasminogen-Free, Bovine
• CAS Number: 9002-04-4
• UNSPSC Code: 12352204
• NACRES: NA.25

Properties

• form: lyophilized
• Quality Level: 100
• specific activity: ≥1000 NIH units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated
• solubility: water: 1 mg/mL
• foreign activity: Plasminogen, none detected (by rocket IEP)
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native, bovine thrombin. Key enzyme that activates factor XIII and converts fibronogen into fibrin. Note: 1 KU = 1000 units.

Native, bovine thrombin. Key enzyme that activates factor XIII and converts fibronogen into fibrin. Serine protease formed in the plasma during the coagulation process by activation of prothrombin by a complex of Factor Xa, Factor Va, phospholipid, and Ca²⁺. Thrombin cleaves Arg-Gly bonds in specific sequences only. The best characterized activity of thrombin is the conversion of fibrinogen to fibrin in the clotting process. Inhibited by AEBSF (Cat. No. 101500) Antithrombin (Cat. No. 169756), PMSF (Cat. No. 52332), and TLCK (Cat. No. 616382). Note: 1 KU = 1000 units.

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is determined by comparison with a standard curve prepared using the Bureau of Biologics standard thrombin.

Physical form

Lyophilized from 200 mM NaCl, 50 mM sodium citrate, 0.1% PEG-8000, pH 6.5.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.

Analysis Note

Complete activation from homogeneous bovine prothrombin by SDS-PAGE

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable