609312

Thyroglobulin, Human Thyroid

• Synonym(s): Thyroglobulin, Human Thyroid
• CAS Number: 9010-34-8
• MDL number: MFCD00077045
• UNSPSC Code: 12352202
• NACRES: NA.25

Properties

• biological source: human thyroid
• Quality Level: 100
• assay: ≥95% (PAGE)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Native thyroglobulin, consisting of two polypeptide monomers of 330 kDa each, purified from human thyroid. This protein is an excellent source of N-linked glycans of various structures, including sialylated core fucose and oligomannose oligosaccharides. Also, Gal-3-SO₄ and GalNAc-6-SO₄ residues located in a single multi-branched complex type glycan have been reported in calf thymus and human proteins. When subjected to native PAGE this protein shows a band at

~660 kDa, representing the dimeric form, and another band at ~330 kDa, representing the monomeric form.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 20 mM NH₄HCO₃.

Preparation Note

Prepared from tissues of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Reconstitute in phosphate buffer, containing 150 mM NaCl, pH >7.0.

Other Notes

Rawitch, A.B., et al. 1993. Arch. Biochem. Biophys. 300, 271.
Black, E.G., and Sheppard, M.C. 1991. Clin. Endocrinol. 35, 519.
Spiro, R.G., and Bhoyroo, V.D. 1988. J. Biol. Chem. 263, 14351.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3