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650297

Sigma-Aldrich

Anti-Trypsin Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Rabbit polyclonal antibody was purified by ammonium sulfate precipitation followed by DEAE-cellulose ion exchange chromatography. Recognizes cationic trypsin.
Recognizes cationic trypsin in pancreas tissue. Does not cross-react with other plasma proteins.
This Anti-Trypsin Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoelectrophoresis for the detection of Trypsin.

Immunogen

Human
human pancreatic trypsin

Application

ELISA (≥1:250)

Immunoblotting (1:2000)

Immunoelectrophoresis (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Human pancreas tissue

Other Notes

Does not cross-react with other plasma proteins. Monospecific as determined by immunoelectrophoresis against human pancreatic extract and against human trypsin. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ji Hye Seo et al.
Digestion, 80(1), 40-49 (2009-05-30)
Proteinase-activated receptor-2 (PAR2), which is activated by trypsin, is known to be associated with expression of adhesion molecule integrins. We previously demonstrated that Helicobacter pylori induced the expression of integrin alpha(5) and beta(1) in human gastric epithelial cells. The present
Carlie S Sigel et al.
Cancer cytopathology, 121(8), 459-470 (2013-02-15)
Acinar cell neoplasms of the pancreas are rare but when encountered, the diagnosis is often established based on cytology specimens. Diagnostic accuracy is important because acinar cell carcinomas are aggressive yet may mimic tumors with different outcomes and management. The

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