MilliporeSigma
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70955

Millipore

5% Alkali-soluble Casein

Unique blocking reagent for ultra-low background

Synonym(s):
blocking buffer, immunodetection blocking buffer

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

shipped in

ambient

storage temp.

2-8°C

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This Item
C7594B6429WBAVDCH01
Millipore

Millipore

70955

5% Alkali-soluble Casein

Sigma-Aldrich

Sigma-Aldrich

C7594

Casein Blocking Buffer

storage condition

OK to freeze

storage condition

-

storage condition

-

storage condition

-

shipped in

ambient

shipped in

-

shipped in

-

shipped in

ambient

storage temp.

2-8°C

storage temp.

room temp

storage temp.

room temp

storage temp.

15-25°C

manufacturer/tradename

Novagen®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Immobilon®

General description

Alkali-Soluble Casein is a superior blocking reagent for Western blot applications. This preparation minimizes non-specific background relative to other blocking reagents, such as BSA, gelatin, or non-fat milk. Alkali-Soluble Casein is effective in colorimetric and chemiluminescent detection protocols. Note that due to its high coating properties, detection sensitivity for some antibodies may be lower than with other blocking reagents under the same conditions (although the background is also reduced); the concentration can be optimized for maximal signal:noise ratios. Supplied as a 5% w/v solution; recommended working concentration range is 0.251%.
Unique blocking reagent for ultra-low background

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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