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AB1542

Sigma-Aldrich

Anti-Tyrosine Hydroxylase Antibody

Chemicon®, from sheep

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Synonym(s):
Tyrosine 3-hydroxylase, Tyrosine 3-hydroxylase, tyrosine hydroxylase
eCl@ss:
32160702
NACRES:
NA.41

biological source

sheep

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, mouse

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... TH(7054)

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This Item
AB5986PSAB4502964AB152
Anti-Tyrosine Hydroxylase Antibody Chemicon®, from sheep

AB1542

Anti-Tyrosine Hydroxylase Antibody

Anti-Tyrosine Hydroxylase Antibody Chemicon®, from rabbit

AB152

Anti-Tyrosine Hydroxylase Antibody

antibody form

affinity isolated antibody

antibody form

affinity purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

Quality Level

100

Quality Level

100

Quality Level

-

Quality Level

100

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

Gene Information

human ... TH(7054)

Gene Information

human ... TH(7054)

Gene Information

human ... TH(7054)

Gene Information

human ... TH(7054)
rat ... Th(25085)

species reactivity

rat, mouse

species reactivity

rat

species reactivity

mouse, rat, human

species reactivity

human, mollusk, feline, ferret, Aplysia (marine mollusc), rat, squid, Drosophila, mouse

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General description

Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

Specificity

Tyrosine Hydroxylase (TH, Tyrosine Monooxygenase). Cross-reacts with all mammalian forms tested to date and some non-mammalian forms.

Immunogen

Native tyrosine hydroxylase from rat pheochromocytoma (J. Biol. Chem.,1982, 257:9416-9423).

Application

Anti-Tyrosine Hydroxylase Antibody is an antibody against Tyrosine Hydroxylase for use in IH & WB.
Immunohistochemistry(peroxidase): A previous lot of this antibody was used at 1:1,000 dilution. The antibody gives specific labeling of noradrenergic axons in primate cerebral cortex (Brain Res., 1989, 500:313-324.).
Note: It is important that you use a secondary antibody that has significant reactivity to sheep IgG1 since the majority of the sheep immunoglobulin is IgG1.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Quality

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of mouse brain lysates.

Target description

~60 kDa

Physical form

ImmunoAffinity Purified
Purified sheep polyclonal in buffer containing 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Storage and Stability

Stable for 6 months at -20 to -80°C in undiluted aliquots from date of receipt. Store in a NON-self-defrosting freezer.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
Positive: brain (corpus striatum) and adrenal gland. Negative: liver.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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Selective optogenetic activation of rostral ventrolateral medullary catecholaminergic neurons produces cardiorespiratory stimulation in conscious mice.
Abbott, SB; DePuy, SD; Nguyen, T; Coates, MB; Stornetta, RL; Guyenet, PG
The Journal of Neuroscience null
Neuropeptide y gates a stress-induced, long-lasting plasticity in the sympathetic nervous system.
Wang, Q; Wang, M; Whim, MD
The Journal of Neuroscience null
Cholinergic neurons in the mouse rostral ventrolateral medulla target sensory afferent areas.
Stornetta, RL; Macon, CJ; Nguyen, TM; Coates, MB; Guyenet, PG
Brain Structure &Amp; Function null
The atypical homeoprotein Pbx1a participates in the axonal pathfinding of mesencephalic dopaminergic neurons.
Sgado, P; Ferretti, E; Grbec, D; Bozzi, Y; Simon, HH
Neural Dev. null
Transsynaptic activity-dependent regulation of axon branching and neurotrophin expression in vivo.
Calinescu, AA; Liu, T; Wang, MM; Borjigin, J
The Journal of Neuroscience null

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