MilliporeSigma
All Photos(1)

AB1551

Sigma-Aldrich

Anti-Metabotropic Glutamate Receptor 1α Antibody, pain, CT

Chemicon®, from rabbit

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
mGluR1alpha
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, monkey, human

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GRM1(2911)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
AB9448AB5675AB9209
antibody form

affinity purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity purified immunoglobulin

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

rat, monkey, human

species reactivity

human

species reactivity

rat, mouse

species reactivity

human

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin)

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

Specificity

Recognizes metabotropic glutamate receptor 1a (mGluR1a). On western blots of rat brain microsomes, the antibody labels a single band migrating at 140 kDa, corresponding to mGluR1alpha (Testa, 1998). The antibody recognizes a 140 kDa band in extracts from transfected cells expressing mGluR1alpha but not in extracts from transfected cells expressing mGluR5 (Marino, 2001).

Immunogen

Carboxy terminal peptide of rat mGluR1alpha conjugated to KLH with glutaraldehyde (PNVTYASVILRDYKQSSSTL).
Epitope: C-terminus

Application

Anti-Metabotropic Glutamate Receptor 1α Antibody, pain, C-terminus is an antibody against Metabotropic Glutamate Receptor 1α for use in IH(P), IH & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Western blot: 0.25-1.0 μg/mL. Detects a band migrating at approximately 140 kDa. The use of membrane preparations and protease inhibitors is highly recommended.

Immunohistochemistry: AB1551 can be used for immunocytochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations recommended are 0.5-2.0 μg/mL.

The antibody has not been tested for use in immunoprecipitation. However, antibodies to the carboxy termini of other glutamate receptors are very effective in immunoprecipitation.

Optimal working dilutions must be determined by end user.

Target description

140 kDa

Linkage

Replaces: AB1595

Physical form

Affinity Purified using the immunogen peptide. Lyophilized from PBS containing 1% BSA.
ImmunoAffinity Purified

Storage and Stability

Maintain lyophilized material at 2–8°C or -20°C. Reconstitute to 500 μL with sterile distilled water. Store reconstituted material frozen (-20°C or -80°C) in undiluted aliquots for up to six months. Avoid repeated thawing and refreezing. Diluted antibody containing 0.1% sodium azide for Western blot analysis can be stored for several weeks and reused repeatedly.

Analysis Note

Control
Cerebellar tissue sections or homogenates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Co-localization of caldesmon and calponin with cortical afferents, metabotropic glutamate and neurotrophic receptors in the lateral and central nuclei of the amygdala.
Agassandian, K; Cassell, MD
Brain Research null
Ultrastructural relationships between cortical, thalamic, and amygdala glutamatergic inputs and group I metabotropic glutamate receptors in the rat accumbens.
Mitrano, DA; Pare, JF; Smith, Y
The Journal of Comparative Neurology null
Up-regulation of hippocampal metabotropic glutamate receptor 5 in temporal lobe epilepsy patients.
Notenboom, RG; Hampson, DR; Jansen, GH; van Rijen, PC; van Veelen, CW; van Nieuwenhuizen et al.
Brain null
D A Mitrano et al.
Neuroscience, 154(2), 653-666 (2008-05-16)
There is significant pharmacological and behavioral evidence that group I metabotropic glutamate receptors (mGluR1a and mGluR5) in the nucleus accumbens play an important role in the neurochemical and pathophysiological mechanisms that underlie addiction to psychostimulants. To further address this issue
Johannes Meek et al.
The Journal of comparative neurology, 510(4), 396-421 (2008-07-30)
This study used immunohistochemistry, Golgi impregnation, and electron microscopy to examine the circuitry of the cerebellum of mormyrid fish. We used antibodies against the following antigens: the neurotransmitters glutamate and gamma-aminobutyric acid (GABA); the GABA-synthesizing enzyme glutamic acid decarboxylase (GAD);

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service