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AB1582

Sigma-Aldrich

Anti-Choline Acetyltransferase Antibody

serum, Chemicon®

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Synonym(s):
ChAT
eCl@ss:
32160702
NACRES:
NA.41

biological source

sheep

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, guinea pig, rabbit

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

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This Item
AB5042MAB5270AB5042P
biological source

sheep

biological source

rabbit

biological source

mouse

biological source

rabbit

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

UniProt accession no.

P28329

UniProt accession no.

P28329

UniProt accession no.

P28329

UniProt accession no.

P28329

Gene Information

rabbit ... Chat(100342226)
rat ... Chat(290567)

Gene Information

chicken ... Chat(395314)
rat ... Chat(290567)

Gene Information

human ... CHAT(1103)
pig ... Chat(396896)
rat ... Chat(290567)

Gene Information

rat ... Chat(290567)

clone

polyclonal

clone

polyclonal

clone

1.B3.9B3, monoclonal

clone

polyclonal

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Specificity

Choline Acetyltransferase (ChAT). Will stain cholinergic neurons in rat, guinea-pig and rabbit central and peripheral nervous systems.

Immunogen

A 22 amino acid peptide from porcine ChAT, coupled to KLH. The sequence is H-GLFSSYRLPGHTQDTLVAQKSS-NH2.

Application

Immunohistochemistry: 1:1,000-1:2,000

Immunoblotting

Optimal working dilutions must be determined by end user.

IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1582

STAINING OF GUINEA PIG ILEUM

1) Pin the ileum out flat and tight.

2) Fix overnight at 4°C in modified Zamboni′s (2% paraformaldehyde with 15% saturated picric acid in phosphate buffered saline).

3) Clear in DMSO, 100%, 3 times for 10 minutes each, rinse in PBS.

4) Dissect off ALL circular muscle.

5) Preincubate with 10% normal donkey serum.

6) Incubate in anti-ChAT (AB1582) diluted 1:1,000-1,2000 with 10% normal donkey serum over night at 4°C on a shaker.

7) Incubate with Donkey anti-Sheep IgG-Cy3 labeled secondary antibody (Catalog Number AP184C) at a dilution of 1:200 for 2 hours.

IMPORTANT NOTE: It is essential to remove ALL circular muscle.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers
This Anti-Choline Acetyltransferase Antibody is validated for use in IH, WB for the detection of Choline Acetyltransferase.

Physical form

Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any residue.

Storage and Stability

Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids


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Guy R Sander et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 51(7), 969-972 (2003-06-18)
The Notch signaling pathway is a vitally important pathway in regulating brain development. To explore the involvement of the Notch pathway in neuronal cells of adult rat gut, we investigated the expression of Notch1 and Jagged2 by in situ hybridization
E E Hur et al.
Neuroscience, 164(4), 1721-1731 (2009-09-26)
The basal forebrain (BF) comprises morphologically and functionally heterogeneous cell populations, including cholinergic and non-cholinergic corticopetal neurons that are implicated in sleep-wake modulation, learning, memory and attention. Several studies suggest that glutamate may be among inputs affecting cholinergic corticopetal neurons
Neural pathways underlying lactate-induced panic.
Johnson, PL; Truitt, WA; Fitz, SD; Lowry, CA; Shekhar, A
Neuropsychopharmacology null
S D Motts et al.
Neuroscience, 154(1), 186-195 (2008-01-29)
We used an antibody to choline acetyltransferase (ChAT) to label cholinergic cells in guinea pig brainstem. ChAT-immunoreactive (IR) cells comprise several prominent groups, including the pedunculopontine tegmental nucleus, laterodorsal tegmental nucleus, and parabigeminal nucleus, as well as the cranial nerve
Cardiac Dysregulation and Myocardial Injury in a 6-Hydroxydopamine-Induced Rat Model of Sympathetic Denervation.
Jiang, YH; Jiang, P; Yang, JL; Ma, DF; Lin, HQ; Su, WG; Wang, Z; Li, X
Testing null

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