MilliporeSigma
All Photos(6)

Documents

AB175

Sigma-Aldrich

Anti-GABA Antibody

serum, Chemicon®

Sign Into View Organizational & Contract Pricing

eCl@ss:
32160702
NACRES:
NA.41

biological source

guinea pig

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
ABN131A2052SAB4200721
vibrant-m

AB175

Anti-GABA Antibody

vibrant-m

ABN131

Anti-GABA Antibody

antibody form

serum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

biological source

guinea pig

biological source

rabbit

biological source

rabbit

biological source

mouse

species reactivity

rat

species reactivity

rat

species reactivity

rat, Drosophila, wide range

species reactivity

rat, monkey, frog, mouse, human, gerbil

technique(s)

ELISA: suitable, immunohistochemistry: suitable (paraffin)

technique(s)

ELISA: suitable, immunohistochemistry: suitable (paraffin)

technique(s)

dot blot: 1:10,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5 μg/mL using rat cerebellum

technique(s)

ELISA: suitable, dot blot: suitable, immunofluorescence: 5-10 μg/mL using human pancreatic tumor AsPC1 cell line, immunohistochemistry: 1-2.5 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human brain and/or cerebellum sections

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

GB-69, monoclonal

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system that inhibits the generation of the action potential of the neuron. It is involved in the pathogenesis of certain neurological and psychiatric disorders. GABA is produced from glutamic acid in a reaction catalyzed by glutamic acid decarboxylase. In this reaction pyridoxal phosphate serves as a co-factor. GABA interacts with the GABAA and GABAB receptors, which are widely distributed throughout the nervous system and in a variety of cell types. They differ in their pharmacological, electrophysiological, and biochemical properties. GABAA-receptor complex mediates an increase in membrane conductance with an equilibrium potential near the resting level of −70 mV. This conductance increase often is accompanied by a membrane hyperpolarization, which later results in a reduction in the probability of action potential initiation. The reduction in membrane resistance is accomplished by the GABA-dependent facilitation of Cl− ion influx. GABAB receptors are coupled indirectly to K+ channels. When activated, GABAB receptors reduce Calcium ion conductance and inhibit cAMP production via intracellular mechanisms mediated by G-proteins. GABAB receptors are known to mediate both postsynaptic and presynaptic inhibition.

Specificity

GABA is a neurotransmitter synthesized in all species, and is therefore expected to react to all species.
Recognizes GABA. Staining was blocked by preabsorbing with 100 μM GABA conjugated to glutaraldehyde. 500 μM of similar conjugations of glutamic acid, glutamate and taurine failed to block staining.

Immunogen

BSA-conjugated GABA-Glutaraldehyde

Application

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected GABA in rat brain tissue.

ELISA Analysis: A representative lot from an independent laboratory detected GABA in a competitive ELISA.

Immunohistochemistry Protocol:

Tissues fixed with 4% paraformaldehyde and 0-0.5% glutaraldehyde gives good results. Glutaraldehyde is required for antibody reactivity.

1) Tissue is fixed with 4% paraformaldehyde, 0-0.5% glutaraldehyde, 0.5% potassium dichromate in 0.1M phosphate buffer at pH 6.5.

2) Tissue is post-fixed overnight, vibratome sectioned in 50 mm and incubated in 0.05M Tris buffer, pH 6.5 for three hours.

3) Sections are incubated for 18-24 hours in AB175 diluted in PBS containing 0.1% sodium azide, 0.2% Triton X-100 and 1% normal goat serum.

4) Fluorescein conjugated antibody or ABC system may be used as the secondary reagent.

Note: Without colchicine pretreatment well-stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchicine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
This Anti-GABA Antibody is validated for use in Immunohistochemistry (Paraffin) and Enzyme Immunoassay (ELISA) for the detection of GABA.

Quality

Evaluated by Immunohistochemistry in rat brain tissue.

Immunohistochemistry Analysis: A 1:500 dilution of this antibody detected GABA in rat brain tissue.

Physical form

Depleted serum
Guinea Pig polyclonal BSA-depleted antiserum with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Rat Brain tissue

Other Notes

Concentration: Variable

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Ji-Jie Pang et al.
Investigative ophthalmology & visual science, 52(7), 4886-4896 (2011-04-13)
To examine the specificity and reliability of a retrograde double-labeling technique that was recently established for identification of retinal ganglion cells (GCs) and to characterize the morphology of displaced (d)GCs (dGs). A mixture of the gap-junction-impermeable dye Lucifer yellow (LY)
Kristina D Micheva et al.
eNeuro, 5(5) (2018-11-09)
Numerous types of inhibitory neurons sculpt the performance of human neocortical circuits, with each type exhibiting a constellation of subcellular phenotypic features in support of its specialized functions. Axonal myelination has been absent among the characteristics used to distinguish inhibitory
Mei Chen et al.
PloS one, 8(4), e61381-e61381 (2013-05-03)
Previous studies have shown that CCL2/CX3CR1 deficient mice on C57BL/6N background (with rd8 mutation) have an early onset (6 weeks) of spontaneous retinal degeneration. In this study, we generated CCL2(-/-)CX3CR1(GFP/GFP) mice on the C57BL/6J background. Retinal degeneration was not detected
Response features of parvalbumin-expressing interneurons suggest precise roles for subtypes of inhibition in visual cortex.
Runyan, CA; Schummers, J; Van Wart, A; Kuhlman, SJ; Wilson, NR; Huang, ZJ; Sur, M
Neuron null
Luca Bragina et al.
Journal of neurochemistry, 105(5), 1781-1793 (2008-02-06)
gamma-Aminobutyric acid 1 (GAT-1) is the most copiously expressed GABA transporter; we studied its role in phasic and tonic inhibition in the neocortex using GAT-1 knockout (KO) mice. Immunoblotting and immunocytochemical studies showed that GAT-2 and GAT-3 levels in KOs

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service