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AB1832P

Sigma-Aldrich

Anti-Interleukin-1β Antibody

Chemicon®, from rabbit

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Synonym(s):
IL-1beta
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
neutralization: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

rat ... Il1B(24494)

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I3642MABF18AV54324
vibrant-m

AB1832P

Anti-Interleukin-1β Antibody

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

biological source

rabbit

biological source

mouse

biological source

mouse

biological source

rabbit

technique(s)

ELISA: suitable, western blot: suitable, neutralization: suitable

technique(s)

flow cytometry: suitable, immunohistochemistry: 8-25 μg/mL using human PBMC′s, neutralization: suitable, western blot: 1 μg/mL

technique(s)

western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

species reactivity

rat

species reactivity

human

species reactivity

human

species reactivity

human

UniProt accession no.

P01584

UniProt accession no.

P01584

UniProt accession no.

P01584

UniProt accession no.

P01584

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Specificity

Reacts with rat Interleukin-1beta (IL-1beta).

Immunogen

Highly pure (>98%) recombinant rat IL-1beta.

Application

Anti-Interleukin-1β Antibody is an antibody against Interleukin-1β for use in ELISA, WB, NEUT.
ELISA: 0.5 μg/mL this dilution will allow detection (in direct ELISA) of

0.2-0.4 ng/well when rat IL-1(is used to coat the plate.

Western blot: 0.1-0.2 μg/mL, this dilution should offer detection limit of 1.5-3.0 ng/lane, under reducing or non-reducing conditions.

Neutralization: to yield ½ maximal inhibition (ND50) of the biological activity of rat IL-1beta (0.2 ng/mL), a concentration of 0.09-0.14 μg/mL of antibody is required.

Final working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors

Linkage

Replaces: 04-462; MABF22

Physical form

Affinity purified Immunoglobulin. Lyophilized from 0.5X PBS pH 7.4, no preservatives. Reconstitute in sterile distilled water.

Storage and Stability

Maintain lyophilized material desiccated below 0°C. After reconstitution maintain at -20°C in convenient undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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PLoS Pathogens null
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To study the molecular mechanisms involved in the hepatoprotective effects of naringenin (NAR) on carbon tetrachloride (CCl4)-induced liver fibrosis. Thirty-two male Wistar rats (120-150 g) were randomly divided into four groups: (1) a control group (n = 8) that received

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