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AB19027

Sigma-Aldrich

Anti-ADAM 17 Antibody

Chemicon®, from rabbit

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Synonym(s):
A disintegrin and metalloproteinase domain 17, ADAM metallopeptidase domain 17, ADAM metallopeptidase domain 17 (tumor necrosis factor, alpha,converting enzyme), CD156b antigen, Snake venom-like protease, TNF-alpha convertase, TNF-alpha converting enzyme
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse

species reactivity (predicted by homology)

rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ADAM17(6868)

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MABT388SAB4502912HPA010738
vibrant-m

AB19027

Anti-ADAM 17 Antibody

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

UniProt accession no.

P78536

UniProt accession no.

P78536

UniProt accession no.

P78536

UniProt accession no.

P78536

clone

polyclonal

clone

16C11.1, monoclonal

clone

polyclonal

clone

polyclonal

Gene Information

human ... ADAM17(6868)

Gene Information

human ... ADAM17(6868)

Gene Information

human ... ADAM17(6868)

Gene Information

human ... ADAM17(6868)

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General description

ADAM17, also known as TNF-alpha Convertase, or TACE, was first cloned from human epithelial cells. Tumor-necrosis factor alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-alpha is synthesized as a 26 kDa Type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-alpha. TNF-alpha converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately (1-3). The ADAM proteins are structurally similar, with a signal sequence, metalloprotease domain (inactive in some ADAMs), disintegrin domain, cystein rich domain, EGF like repeat, type I transmembrane, and a cytoplasmic domain. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), ADAM17 is known to process the TNF alpha trimer from the membrane attached precursor to the soluble form. ADAM17 contains the canonical HExxHxxxxxH zinc metallo-proteinase motif, and has been shown to be proteolytically active, cleaving TNF precursor as well as TNF p75 receptor, myeloid precursor protein, amyloid plaque protein, L selectin and TGF alpha, making ADAM17 a "sheddase". Reports have shown that ADAM17 expression in human breast tumor samples is an indicator of poor prognosis.

Specificity

TACE is a membrane-bound metallo-proteinase-disintegrin. ADAM 17 also processes other cell surface proteins, including TNF receptor, TGF alpha, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP) (4,5). ADAM 17 mRNA is expressed in a variety of human and murine tissues.

Immunogen

Raised against a peptide corresponding to amino acids 807-823 of human TACE (1,2). This sequence differs from those of mouse and rat TACE by one amino acid.(3).

Application

Detect ADAM 17 using this Anti-ADAM 17 Antibody validated for use in Western blotting and Immunocytochemistry.
Research Category
Cell Structure
Research Sub Category
MMPs & TIMPs
Western blot:
1:500 - 1:2,000 dilution from a previous lot was used. HeLa or Jurkat whole cell lysate can be used as a positive control and 80 to 130 kDa bands can be detected which may represent mature protein, precursor, and glycosylated TACE.

Immunocytochemistry:

Optimal working dilutions must be determined by end user.

Quality

Routinely evaluated by Immunocytochemistry on A431 cells.

Immunocytochemistry Analysis: Confolcal fluorescent analysis of A431 using rabbit anti-ADAM 17 polyclonal antibody.

Target description

80 to 130 kDa

Physical form

Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing PBS and 0.02% sodium azide.

Storage and Stability

Stable 1 year at -20°C in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Human Cell lysate - whole cell (MDA-MB231 Cell Line)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Sayantan Jana et al.
Journal of the American Heart Association, 7(19), e010427-e010427 (2018-10-30)
Background Dilated cardiomyopathy (DCM) is a common cause of heart failure in adult and pediatric patients, but the underlying mechanism may vary in adults and children, with few studies conducted to date. The objective of the present study was to
Peter Thornton et al.
EMBO molecular medicine, 9(10), 1366-1378 (2017-09-01)
We have characterised the proteolytic cleavage events responsible for the shedding of triggering receptor expressed on myeloid cells 2 (TREM2) from primary cultures of human macrophages, murine microglia and TREM2-expressing human embryonic kidney (HEK293) cells. In all cell types, a
Anna Maria Kanarek et al.
The FEBS journal, 284(5), 742-753 (2017-01-20)
The association between hypertension and an increased risk for Alzheimer's disease (AD) and dementia is well established. Many data suggest that modulation of the renin-angiotensin system may be meaningful for the prevention and therapy of neurodegenerative disorders, in particular AD.
Anselm Sommer et al.
Oncotarget, 8(42), 72584-72596 (2017-10-27)
ADAM17, a prominent member of the "Disintegrin and Metalloproteinase" (ADAM) family, is an important regulator of endothelial cell proliferation and cell survival. The protease controls vital cellular functions through cleavage of growth factors, cytokines and their receptors including transforming growth
Snježana Mikuličić et al.
Medical microbiology and immunology, 209(4), 461-471 (2020-05-10)
Human papillomaviruses (HPV) are causative agents of various tumours such as cervical cancer. HPV binding to the cell surface of keratinocytes leads to virus endocytosis at tetraspanin enriched microdomains. Complex interactions of the capsid proteins with host proteins as well

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