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AB5084P

Sigma-Aldrich

Anti-Dopamine D2 Receptor Antibody

Chemicon®, from rabbit

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Synonym(s):
Dopamine D2 receptor
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, monkey, rat, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... DRD2(1813)
mouse ... Drd2(13489)
rat ... Drd2(24318)
rhesus monkey ... Drd2(694181)

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This Item
D2944ABN462AB1792P
Anti-Dopamine D2 Receptor Antibody from rabbit, purified by affinity chromatography

ABN462

Anti-Dopamine D2 Receptor Antibody

Quality Level

100

Quality Level

-

Quality Level

100

Quality Level

100

biological source

rabbit

biological source

rat

biological source

rabbit

biological source

rabbit

technique(s)

ELISA: suitable, immunoprecipitation (IP): suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: 5-10 μg/mL using D1 dopamine receptor transfected HEK-293T cells, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

species reactivity

mouse, monkey, rat, human

species reactivity

rat, monkey, human

species reactivity

mouse

species reactivity

canine, rat

Gene Information

human ... DRD2(1813)
mouse ... Drd2(13489)
rat ... Drd2(24318)
rhesus monkey ... Drd2(694181)

Gene Information

human ... DRD1(1812)
rat ... Drd1a(24316)

Gene Information

mouse ... Drd2(13489)

Gene Information

dog ... Drd2(403701)
rat ... Drd2(24318)

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General description

The Dopamine D2 Receptor is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. 2 isoforms are reported for the D2 Dopamine Receptor, Isoform 1 and isoform 2 which are expressed in the pituitary and brain; Isoform 1 is expressed seven times more than isoform 2 in the caudate nucleus.

Specificity

Specific for Dopamine Receptor D2 short and long form. Dopamine D2 receptor is known to exist in the short (D2S) and the long (D2L) forms that are encoded by splice variants of a single gene and differ only by the presence of an additional 29 amino acid in the intracellular, cytoplasmic loop 3 (1). D2L receptor is a 446 aa, G-protein coupled and transmembrane receptor protein (2). The immunogen peptide shows no significant homology with other dopamine receptors (D1 and D3-D5).

Immunogen

A 28 amino acid peptide sequence from the human D2 receptor within the cytoplasmic
loop #3.

Application

Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
This Anti-Dopamine D2 Receptor Antibody is validated for use in ELISA, IH, IP, WB for the detection of Dopamine D2 Receptor.
Western blot:
1-10 µg/mL (Chemiluminescence technique) The predicted size of D2 is approximately 50 kDa. However, additional bands at approximately 68 and 100 kDa, and others are typically seen in various tissues (Boundy et al. 1993; Sakata et al. 1992; Farooqui et al. 199; Farooqui & Prasad 1992, BBRC; Brock et al. 1992; Farooqui & Prasad 1992, Life Sci). Approximately 100-400 µg of membrane protein/lane should be loaded to visualize various bands.

Immunohistochemistry:
2-10 μg/mL on paraformaldehyde fixed sections on a previous lot.
Immunohistochemistry:
1-20 µg/mL on paraformaldehyde fixed sections.

Immunoprecipitation:
1-10 μg/mL using 100-400 μg of protein on a previous lot. An antibody made to this epitope has been shown to immunoprecipitate both the long and short forms (2).

ELISA:
0.5-1.0 μg/mL (1 μg/mL immunogen peptide (Catalog number AG221)/well) of a previous lot was used on ELISA.

Optimal working dilutions must be determined by the end user.

Quality

Routinely evaluated by Western Blot on human brain lysates.

Western Blot Analysis: 1:1000 dilution of this lot detected Dopamine Receptor D2 on 10 μg of human brain lysates.

Target description

50 kDa

Physical form

ImmunoAffinity Purified
Purified rabbit polyclonal liquid in PBS with 0.1% BSA.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Monkey brain tissue, rat brain lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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