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D2944

Sigma-Aldrich

Anti-D1 Dopamine Receptor antibody,Rat monoclonal

clone 1-1-F11 s.E6, purified from hybridoma cell culture

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Synonym(s):
Anti-DRD1
MDL number:
UNSPSC Code:
12352203
NACRES:
NA.25

biological source

rat

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1-1-F11 s.E6, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

rat, monkey, human

concentration

~1 mg/mL

technique(s)

immunocytochemistry: 5-10 μg/mL using D1 dopamine receptor transfected HEK-293T cells
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DRD1(1812)
rat ... Drd1a(24316)

General description

Monoclonal Anti-D1 Dopamine Receptor (rat IgG2a isotype) is derived from the rat hybridoma 1-1-F11 S.E6 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with recombinant fusion protein containing the C-terminal 97 amino acid of human D1 dopamine receptor. Dopamine receptors belong to the family of seven transmembrane domain G protein coupled receptors (GPCRs). Dopamine receptors are mainly localized in the striatum, limbic system, the brain cortex and the infundibulum. The D1-like family consists of the D1 and D5 receptors.

Immunogen

recombinant fusion protein containing the C-terminal 97 amino acid of human D1 dopamine receptor.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-D1 Dopamine Receptor antibody produced in rat has been used in:
  • immunohistochemistry
  • immunoblotting
  • immunocytochemistry
  • proximity ligation assay
  • co-immunoprecipitation
  • western blotting

Biochem/physiol Actions

D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. D1 receptors are expressed on airway smooth muscle (ASM) and regulate smooth muscle force via cAMP activation of protein kinase A. It is a potential target for therapeutic relaxation of ASM.

Target description

D1 Dopamine Receptor encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

Sold under exclusive license from Emory University

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kentaro Mizuta et al.
Respiratory research, 14, 89-89 (2013-09-06)
Dopamine signaling is mediated by Gs protein-coupled "D1-like" receptors (D1 and D5) and Gi-coupled "D2-like" receptors (D2-4). In asthmatic patients, inhaled dopamine induces bronchodilation. Although the Gi-coupled dopamine D2 receptor is expressed and sensitizes adenylyl cyclase activity in airway smooth
Eleanor H Simpson et al.
Biological psychiatry, 76(10), 823-831 (2014-01-07)
Evidence indicating an increase in dopamine D2 receptor (D2R) density and occupancy in patients with schizophrenia comes from positron emission tomography studies using ligands that bind both D2Rs and dopamine D3 receptors (D3Rs), questioning the role of D3Rs in the
Satoshi Goto et al.
Frontiers in neuroanatomy, 9, 22-22 (2015-03-19)
Immunohistochemistry (IHC) is a valuable method for identifying discrete neurochemical molecules by the interaction of target antigens with validated antibodies tagged with a visible label (e.g., peroxidase). We have developed an immunostaining method that is highly sensitive in detection of
Impact of early consumption of high-fat diet on the mesolimbic dopaminergic system
Naneix F, et al.
eNeuro, 4(3) (2017)
D1R/GluN1 complexes in the striatum integrate dopamine and glutamate signalling to control synaptic plasticity and cocaine-induced responses
Cahill E, et al.
Molecular Psychiatry, 19(12), 1295-1295 (2014)

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