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AB5338P

Sigma-Aldrich

Anti-Cocaine and Amphetamine Related Transcript Antibody

Chemicon®, from rabbit

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Synonym(s):
CART/CARP
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

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This Item
AB5340PSAB4301768SAB1405154
Anti-CARTPT affinity isolated antibody

Sigma-Aldrich

SAB4301768

Anti-CARTPT

antibody form

affinity purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

2E6, monoclonal

species reactivity

mouse, rat

species reactivity

mouse, rat

species reactivity

human, rat, mouse

species reactivity

human

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: 1:100-1:200, western blot: 1:200-1:1000

technique(s)

capture ELISA: suitable, indirect ELISA: suitable, indirect immunofluorescence: suitable, western blot: 1-5 μg/mL

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Specificity

Recognizes Cocaine- and Amphetamine Related Transcript (CART). CART has been found to control satiety modulating the actions of two key regulators of food intake, leptin and NPY. Starvation decreases CART levels in the arcuate nucleus (Kristensen et al. 1998). Obese animals have virtually no CART. CART inhibits both normal and starvation-induced feeding, and completely inhibits NPY-induced feeding. Immunoneutralization of CART by administration of anti-CART resulted into higher food intake suggesting that CART is an endogenous regulator of food intake.

Immunogen

Mouse/rat CART peptide (55-102).

Application

Research Category
Neuroscience
Research Sub Category
CNS Control of Metabolism

Reward & Addiction
This Anti-Cocaine & Amphetamine Related Transcript Antibody is validated for use in ELISA, WB, IH for the detection of Cocaine & Amphetamine Related Transcript.
Western blotting: 1-10 μg/mL using ECL.

Immunohistochemistry: 5-10 μg/mL It has been reported that the antibody will recognize CART in neurons (cell bodies) from the rat hypothalamic arcuate nucleus.

ELISA: 1:10,000-1:50,000 using 50-100 ng control peptide (Catalog Number AG228) per well.

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA. No preservative.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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