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AB5360

Sigma-Aldrich

Anti-Potassium Channel Kv4.2 Antibody

Chemicon®, from rabbit

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eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... KCND2(3751)

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AB5178SAB4501622MABN652
antibody form

affinity purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

technique(s)

western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: 1:10000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

K17/70, monoclonal

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General description

This Anti-Potassium Channel Kv4.2 Antibody is validated for use in WB for the detection of Potassium Channel Kv4.2.

Specificity

Recognizes Kv4.2 (Shal1, RK5, Kcnd2). The epitope specific for Kv4.2 is not present in any other known proteins.

SPECIES REACTIVITIES: Rat. Reactivity with other species has not yet been tested. It is expected to work on mouse and human because the immunogen sequence is highly homologous (15/16 residues identical).

Immunogen

Highly purified peptide corresponding to residues 454-469 of rat Kv4.2 (Accession Q63881).

Application

Anti-Potassium Channel Kv4.2 Antibody is an antibody against Potassium Channel Kv4.2 for use in WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western blotting: 1:200 using ECL on rat brain membranes.

Immunohistochemistry on rat brain sections.

Immunoprecipitation Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Target description

70 kDa

Physical form

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, and 0.025% sodium azide. Reconstitute with 50 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Storage and Stability

Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

hcodes

Hazard Classifications

Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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Wei Zhou et al.
Neuron, 41(4), 573-586 (2004-02-26)
Four Kv channel-interacting proteins (KChIP1 through KChIP4) interact directly with the N-terminal domain of three Shal-type voltage-gated potassium channels (Kv4.1, Kv4.2, and Kv4.3) to modulate cell surface expression and function of Kv4 channels. Here we report a 2.0 Angstrom crystal
Daniel I Levy et al.
The Journal of physiology, 588(Pt 14), 2657-2668 (2010-05-26)
MiRP3, the single-span membrane protein encoded by KCNE4, is localized by immunofluorescence microscopy to the transverse tubules of murine cardiac myocytes. MiRP3 is found to co-localize with Kv4.2 subunits that contribute to cardiac transient outward potassium currents (I(to)). Whole-cell, voltage-clamp
Henry H Jerng et al.
The Journal of physiology, 568(Pt 3), 767-788 (2005-08-27)
Kv4 pore-forming subunits are the principal constituents of the voltage-gated K+ channel underlying somatodendritic subthreshold A-type currents (I(SA)) in neurones. Two structurally distinct types of Kv4 channel modulators, Kv channel-interacting proteins (KChIPs) and dipeptidyl-peptidase-like proteins (DPLs: DPP6 or DPPX, DPP10
Jochen Rose et al.
American journal of physiology. Heart and circulatory physiology, 288(5), H2077-H2087 (2005-01-08)
Action potential (AP) prolongation is a hallmark of failing myocardium. Functional downregulation of K currents is a prominent feature of cells isolated from failing ventricles. The detailed changes in K current expression differ depending on the species, the region of

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