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AB5475

Sigma-Aldrich

Anti-Prox 1 Antibody

serum, Chemicon®

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Synonym(s):
Anti-A230003G05Rik, Anti-PROX-1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, mouse, zebrafish

species reactivity (predicted by homology)

human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... PROX1(5629)

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vibrant-m

AB5475

Anti-Prox 1 Antibody

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

100

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

rabbit

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable

technique(s)

flow cytometry: 1:25, immunohistochemistry: 1:50-1:100, western blot: 1:2000

technique(s)

immunocytochemistry: suitable, indirect ELISA: suitable, indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HepG2 cells, western blot: suitable

technique(s)

western blot: 1:500-1:2000

UniProt accession no.

Q92786

UniProt accession no.

Q92786

UniProt accession no.

Q92786

UniProt accession no.

Q92786

species reactivity

rat, mouse, zebrafish

species reactivity

human, mouse

species reactivity

chicken, rat, lizard, human

species reactivity

human, rat, mouse

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General description

Prox1, the homolog of Drosophila Prospero, is a homeobox-containing transcription factor that binds and functions as a co-receptor of liver receptor homolog 1 (LRH1/NR5A2). It is a specific marker for lymphatic endothelial cells, as well as hepatoblasts and hepatocytes. Human Prox1 shares 97% amino acid sequence identity with mouse and rat Prox1.

Specificity

Other species have not been tested.
Recognizes Prox1. The antibody is useful for identifying Prox1 expressing cells during the development of many areas of the nervous system including the dorsal spinal cord, ganglionic eminences, thalamus and hippocampus.

Immunogen

Synthetic peptide from the C-terminus of mouse Prox1.

Application

Detect Prox 1 using this Anti-Prox 1 Antibody validated for use in IC, IH.
Immunohistochemistry:
1:2,000-1:10,000 with overnight incubation on 4% paraformaldehyde fixed, frozen tissue sections. For embryos immersion fixation is recommended. For older animals perfusion fixation is recommended. Suggested permeabilization solution is 0.1% Triton X100. Suggested blocking buffer is PBS with 0.1% Triton and 10% serum.

Immunocytochemistry:
A previous lot of this antibody was used on Immunocytochemistry

Physical form

Rabbit serum in 50 % glycerol.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
Embryonic brain tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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Decrease in tonic inhibition contributes to increase in dentate semilunar granule cell excitability after brain injury.
Gupta, A; Elgammal, FS; Proddutur, A; Shah, S; Santhakumar, V
The Journal of Neuroscience null
Notum homolog plays a novel role in primary motor innervation.
Cantu, JA; Flowers, GP; Topczewski, J
The Journal of Neuroscience null
The ubiquitin proteasome system is required for cell proliferation of the lens epithelium and for differentiation of lens fiber cells in zebrafish.
Imai, F; Yoshizawa, A; Fujimori-Tonou, N; Kawakami, K; Masai, I
Development null
Granule cells in the CA3 area.
Szabadics, J; Varga, C; Brunner, J; Chen, K; Soltesz, I
The Journal of Neuroscience null
Zhiyong Liu et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 241(4), 684-696 (2012-02-23)
Notch signaling is active in mouse cochlear prosensory progenitors but declines in differentiating sensory hair cells (HCs). Overactivation of the Notch1 intracellular domain (NICD) in progenitors blocks HC fate commitment and/or differentiation. However, it is not known whether reactivation of

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