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Anti-Recoverin Antibody

from rabbit, purified by affinity chromatography

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Cancer-associated retinopathy protein, Cancer-associated retinopathy antigen, Protein CAR, Recoverin

biological source


Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies



purified by

affinity chromatography

species reactivity

human, rat

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)


immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification


Gene Information

mouse ... Rcvrn(19674)

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Anti-Hec1 Antibody from rabbit, purified by affinity chromatography



Anti-Hec1 Antibody

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

affinity isolated antibody




6B12, monoclonal


1K10, monoclonal, recombinant monoclonal



species reactivity

human, rat

species reactivity

porcine, bovine, human, rat, mouse

species reactivity

human, bovine

species reactivity



immunohistochemistry: suitable, western blot: suitable


immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable


affinity binding assay: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable


immunocytochemistry: suitable, western blot: suitable

NCBI accession no.


NCBI accession no.


NCBI accession no.


NCBI accession no.


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General description

Recoverin (UniProt P35243; also known as Cancer-associated retinopathy antigen, Cancer-associated retinopathy protein, Protein CAR) is encoded by the RCVRN (also known as RCV1) gene (Gene ID 5957) in human. The photoreceptor protein recoverin is a member of the EF-hand neuronal calcium sensor (NCS) proteins. Recoverin is initially produced as a 200-amino acid protein whose N-terminal initiator methionine is removed posttranslationally. Recoverin is composed of four EF-hand domains (a.a. 25-60, 61-96, 97-132, 147-182), with the second and third EF-hand regions mediating low- and high-affinity calcium binding, respectively. In photoreceptor cells, recoverin functions as a Ca2+-dependent inhibitor of G-protein-coupled receptor kinase 1 (GRK1). Recoverin plays an important role in mediating calcium-dependent rod and cone light adaptation. Recoverin delays the rod & cone response under dim light when [Ca2+] is high by inhibiting GRK1-catalyzed visual pigment phosphorylation, but has little effect during light adaptation when [Ca2+]i is lowered.


Epitope: EF-hand 4
KLH-conjugated linear peptide corresponding to a sequence from the EF-hand 4 domain of human recoverin.


Anti-Recoverin Antibody detects level of Recoverin and has been published and validated for use in Western Blotting, Immunohistochemistry.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Recoverin in human and rat retina tissue sections.
Research Category
Research Sub Category
Sensory & PNS


Evaluated by Western Blotting in human retina tissue lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected Recoverin in 10 µg of human retina tissue lysate.

Target description

~23 kDa observed.

Physical form

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Biju B Thomas et al.
Frontiers in neuroscience, 15, 752958-752958 (2021-11-13)
End-stage age-related macular degeneration (AMD) and retinitis pigmentosa (RP) are two major retinal degenerative (RD) conditions that result in irreversible vision loss. Permanent eye damage can also occur in battlefields or due to accidents. This suggests there is an unmet
Bin Lin et al.
Investigative ophthalmology & visual science, 61(11), 34-34 (2020-09-19)
To study if human embryonic stem cell-derived photoreceptors could survive and function without the support of retinal pigment epithelium (RPE) after transplantation into Royal College of Surgeons rats, a rat model of retinal degeneration caused by RPE dysfunction. CSC14 human

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