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AB1783

Sigma-Aldrich

Anti-Glutamate Transporter Antibody, Glial

serum, Chemicon®

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Synonym(s):
GLT-1, EAAT2
eCl@ss:
32160702
NACRES:
NA.41

biological source

guinea pig

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SLC1A2(6506)

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This Item
MAB2262SAB1404373AB5905
biological source

guinea pig

biological source

mouse

biological source

mouse

biological source

guinea pig

antibody form

serum

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

serum

species reactivity

human, mouse, rat

species reactivity

mouse, human

species reactivity

human

species reactivity

rat

technique(s)

immunofluorescence: suitable, western blot: suitable, immunohistochemistry: suitable (paraffin)

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

capture ELISA: suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL

technique(s)

immunohistochemistry: suitable

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

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General description

Glutamate transporters (GluT) function to remove L-glutamate (Glu), the primary excitatory neurotransmitter in the mammalian central nervous system (CNS), from the synaptic cleft. By clearing the synapse in this manner Glu can be recycled for later use, the proper diffusion gradient can be maintained and excitotoxicity can be prevented. There have been reports of many different members of a GluT multigene family; GLT1, GLAST, EAAC1 and EAAT2. mRNA has been identified in brain for each of these proteins.

EAAT2 transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

Specificity

Glial Glutamate Transporter GLT-1 (EAAT2). The antibody has been tested on central nervous system tissue. Preabsorption of the antiserum with the immunogen peptide (Catalog number AG391) completely abolishes the immunostaining.

Immunogen

Epitope: C-terminus
Synthetic peptide from the carboxy-terminus of rat GLT-1.

Application

Detect Glutamate Transporter using this Anti-Glutamate Transporter Antibody, Glial validated for use in IH(P), IF & WB.
Evaluated by Western Blot on mouse brain membrane lysates.

Western Blot: 1:500 dilution of this antibody detected GLT-1 on 10 ug of mouse brain membrane lysates.

Immunohistochemistry:
1:1,000-1:4,000 dilution from a previuos lot used a DAB detection system on adult rat forebrain fixed with 4% paraformaldehyde.

Immunohistochemistry:
1:5,000-10,000 dilution from a previuos lot used a cyanine conjugated secondary antibody.

Enzymatic detection requires substantially higher primary antibody dilutions. Lightly fixed 4% PFA material is recommended.

Notes:

Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with 50 mL of Ca2+-free Tyrode+s solution followed by a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) for 6 minutes. Tissues were rapidly dissected out, postfixed in the same fixative for 90 minutes and rinsed for at least 24 hours in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Sections were cut (14 um) in a cryostat and incubated at 4°C overnight with AB1783 (1:5,000-1:10,000). After rinsing in PBS sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.



Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Quality

Evaluated by Western Blot on mouse brain membrane lysates.

Target description

~62 kDa

Physical form

Guinea pig antiserum containing no preservatives.
Unpurified

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Rat brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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Lesion-induced alterations in astrocyte glutamate transporter expression and function in the hippocampus.
Schreiner, AE; Berlinger, E; Langer, J; Kafitz, KW; Rose, CR
ISRN neurology null
Activity of D-amino acid oxidase is widespread in the human central nervous system.
Sasabe, J; Suzuki, M; Imanishi, N; Aiso, S
Frontiers in synaptic neuroscience null
R C Roberts et al.
Neuroscience, 277, 522-540 (2014-07-30)
The process of glutamate release, activity, and reuptake involves the astrocyte, the presynaptic and postsynaptic neurons. Glutamate is released into the synapse and may occupy and activate receptors on both neurons and astrocytes. Glutamate is rapidly removed from the synapse
Noncell-autonomous photoreceptor degeneration in a zebrafish model of choroideremia.
Krock, BL; Bilotta, J; Perkins, BD
Proceedings of the National Academy of Sciences of the USA null
Novel treatment with neuroprotective and antiviral properties against a neuroinvasive human respiratory virus.
Brison, E; Jacomy, H; Desforges, M; Talbot, PJ
Journal of virology null

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