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AB5731

Sigma-Aldrich

Anti-Nanog Antibody, NT

Chemicon®, from rabbit

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Synonym(s):
Homeobox transcription factor Nanog, Nanog homeobox
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat, mouse

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... NANOG(79923)
mouse ... Nanog(71950)

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Anti-Nanog Antibody serum, Chemicon®

Sigma-Aldrich

AB9220

Anti-Nanog Antibody

antibody form

affinity purified immunoglobulin

antibody form

serum

antibody form

purified immunoglobulin

antibody form

purified antibody

clone

polyclonal

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polyclonal

clone

NNG-811, monoclonal

clone

7F7.1, monoclonal

species reactivity

human, rat, mouse

species reactivity

mouse, human

species reactivity

human

species reactivity

human

packaging

antibody small pack of 25 μg

packaging

-

packaging

antibody small pack of 25 μL

packaging

-

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

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General description

NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency, i.e., these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).
NANOG may be useful in the immunohistochemical diagnosis of tumors. NANOG is expressed in germ cells of the fetus and in some germ cell tumors of the gonads and central nervous system (CNS).

Specificity

Antibody recognizes Nanog.

Immunogen

Epitope: N-terminus
Synthetic peptide from mouse Nanog.

Application

Detect Nanog using this Anti-Nanog Antibody, N-terminus validated for use in WB.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Western blotting:
0.5-1.0 µg/mL. The antibody recognizes a protein of ~35 kDa on NT2/D1 cell lysate. Additional bands of ~55 and ~130 kDa may be seen depending on sample and antibody dilution used.Optimal working dilutions must be determined by the end user.

Quality

Routinely evaluated by Western Blot on Embryonic Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected NANOG on 10 μg of Embryonic Mouse Brain lysates

Target description

39 kDa (MW 34 kDa)

Physical form

Affinity purified
Purified rabbit polyclonal immunoglobulin in buffer containing liquid 0.1% sodium azide.

Storage and Stability

Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Whole cell lysate from mouse embryonic stem cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Simple and efficient method for generation of induced pluripotent stem cells using piggyBac transposition of doxycycline-inducible factors and an EOS reporter system.
Tsukiyama T, Asano R, Kawaguchi T, Kim N, Yamada M, Minami N, Ohinata Y, Imai H.
Genes Cells null
Establishing clonal cell lines with endothelial-like potential from CD9(hi), SSEA-1(-) cells in embryonic stem cell-derived embryoid bodies.
Lian, Q; Yeo, KS; Que, J; Tan, EK; Yu, F; Yin, Y; Salto-Tellez, M; Menshawe El Oakley, R; Lim, SK
Testing null
A distinct expression pattern in mammalian testes indicates a conserved role for NANOG in spermatogenesis.
Kuijk, EW; de Gier, J; Lopes, SM; Chambers, I; van Pelt, AM; Colenbrander, B; Roelen, BA
Testing null
Kunie Hagiwara et al.
PloS one, 9(1), e84072-e84072 (2014-01-28)
The outstanding differentiation capacities and easier access from adult tissues, cells derived from neural crest cells (NCCs) have fascinated scientists in developmental biology and regenerative medicine. Differentiation potentials of NCCs are known to depend on their originating regions. Here, we
Gaelen S Smith et al.
Biomolecules, 3(3), 351-368 (2014-03-13)
Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid that activates a family of G protein coupled-receptors (GPCRs) implicated in mammalian development, angiogenesis, immunity and tissue regeneration. S1P functions as a trophic factor for many cell types, including embryonic stem cells (ESCs). Sphingosine

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