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P0056

Sigma-Aldrich

Anti-POU5F1 (Oct4) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-OCT3, Anti-OCT4, Anti-OTF3, Anti-OTF4, Anti-Octamer-binding transcription factor 3, Anti-Pou domain, Class 5, Transcription factor 1

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~43 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using NT2 cell lysate
indirect immunofluorescence: 2-5 μg/mL using paraformaldhyde-fixed F9 cells
western blot: 1-2 μg/mL using NT2 cell lysate

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... POU5F1(5460)
mouse ... Pou5f1(18999)
rat ... Pou5f1(294562)

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This Item
O8389P0082SAB1404228
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

UniProt accession no.

Q01860

UniProt accession no.

Q01860

UniProt accession no.

Q01860

UniProt accession no.

Q01860

technique(s)

immunoprecipitation (IP): 5-10 μg using NT2 cell lysate, western blot: 1-2 μg/mL using NT2 cell lysate, indirect immunofluorescence: 2-5 μg/mL using paraformaldhyde-fixed F9 cells

technique(s)

immunohistochemistry: 1:100-1:250, western blot: 1:500-1:1,000

technique(s)

immunocytochemistry: suitable, indirect ELISA: suitable, western blot: 0.5-1 μg/mL using NT2/D1 total cell extract

technique(s)

indirect ELISA: suitable, western blot: 1-5 μg/mL

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General description

POU class 5 homeobox 1 (POU5F1) recognizes defined octamer DNA element by a shared, conserved DNA-binding domain, namely the POU domain.
POU class 5 homeobox 1 (POU5F1), also known as octamer-binding transcription factor-4 (OCT-4), is expressed in embryonic stem cells and germ cells. It belongs to the POU family of transcription factors. Three splice variants of the protein (OCT4A, OCT4B and OCT4B1) have been identified. The gene encoding this transcription factor is localized on human chromosome 6p21.33.

Immunogen

synthetic peptide corresponding to amino acids 19-34 of human POU5F1, conjugated to KLH. The corresponding sequence in rat is 81% homologous.

Application

Anti-POU5F1 (Oct4) antibody produced in rabbit has been used in Western Blotting.

Biochem/physiol Actions

POU class 5 homeobox 1 (POU5F1) may be a tumor initiating stem-like cell (TSC)-related marker. It has a role in stem cell pluripotency and self-renewal, and also modulates differentiation. The protein has been shown to be expressed in several cancers. POU5F1 is also vital for the induced pluripotent stem cell (iPSC) process.
POU5F1 or Oct4/Oct3 (Octamer-binding transcription factor), functions as an early developmental control gene. It has been recognized as fundamental in the maintenance of pluripotency in embryonic stem cells and in primordial germ cells. It is down regulated in all differentiated somatic cell types both in vivo and in vitro. This is being accomplished through increased DNA methylation and structural changes involving the immediate upstream regulatory region. POU5F1 serves as a marker for germ cell tumors (GCTs) eliciting pluripotentiality, especially including embryonal, seminoma/ dysgerminoma/ germinoma.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Distinctive expression pattern of OCT4 variants in different types of breast cancer.
Soheili S
Cancer Biomarkers : Section A of Disease Markers (2017)
Expression and Prognostic Value of Oct-4 in Astrocytic Brain Tumors
Jeanette K
PLoS ONE (2016)
STAT3 inhibitor enhances chemotherapy drug efficacy by
modulating mucin 1 expression in non-small cell lung
carcinoma
Yunguo Han
Tropical Journal of Pharmaceutical Research (2017)
Huiping Tan et al.
Medical science monitor : international medical journal of experimental and clinical research, 24, 3644-3652 (2018-06-01)
BACKGROUND Recent studies have shown that increased mobilization of Long Interspersed Nuclear Elements-1 (L1) can promote the pathophysiology of multiple neurological diseases. However, its role in Huntington's disease (HD) remains unknown. MATERIAL AND METHODS R6/2 mice - a common mouse
Hepatitis C virus core protein regulates OCT4 expression and promotes cell cycle progression in hepatocellular carcinoma
Jia-Jia Zhou
Oncology Reports (2016)

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