MilliporeSigma
All Photos(11)

Documents

ZRB1101

Sigma-Aldrich

Anti-OCT4 Antibody, clone 4N8, ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

Sign Into View Organizational & Contract Pricing

biological source

rabbit (recombinant)

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

4N8, recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 38.57 kDa
observed mol wt ~42 kDa

species reactivity

human, mouse

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable (peptide)

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... POU5F1(5460)
mouse ... Pou5F1(18999)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
ZRB1151ZRB1105ZRB1098
biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

species reactivity

human, mouse

species reactivity

mouse, human, rat

species reactivity

human, mouse

species reactivity

rat, human, mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

clone

4N8, recombinant monoclonal

clone

5D16, recombinant monoclonal

clone

8G18, recombinant monoclonal

clone

4L5, recombinant monoclonal

Gene Information

human ... POU5F1(5460)
mouse ... Pou5F1(18999)

Gene Information

human ... APP(351)
mouse ... App(11820)

Gene Information

mouse ... Cebpb(12608)

Gene Information

human ... POU4F1(5457)

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 4N8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Octamer-binding protein 4 (OCT4) in human and mouse tissues. It targets an epitope with in the N-terminal region.

Immunogen

His-tagged recombinant fragment corresponding to the first 134 amino acids from human Octamer-binding protein 4 (OCT4).

Application

Anti-OCT4, clone 4N8 ZooMAb , Cat. No. ZRB1101, is a highly specific recombinant rabbit monoclonal antibody that detects OCT4 and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected OCT4 in NTERA2 cell line.

Flow Cytometry Analysis: 1 μg from a representative lot detected OCT4 in NTERA2 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected OCT4 in human testis tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

POU domain, class 5, transcription factor 1 (UniProt: Q01860 also known as Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, OCT4, Octamer-binding transcription factor 3, OTF-3) is encoded by the POU5F1 (also known as OCT3, OCT4, and OTF3) (Gene ID: 5460) in human. OCT4 is a transcription factor that binds to the octamer motif (5′-ATTTGCAT-3′). It forms a trimeric complex with SOX2 on DNA and controls the expression of several genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. It is shown to be critical for early embryogenesis and for embryonic stem cell pluripotency. OCT4 is highly expressed in undifferentiated embryonic stem cells and its expression decreases gradually after embryoid body (EB) formation. In humans two isoforms of OCT4 have been described that are generated by alternative splicing. The isoforms, OCT4-IA (360 amino acids) and OCT4-IB (265 amino acids) have identical C-terminal (225 amino acids), but differ in sequence at their N- terminal region. It has been reported that OCT4-IA is essential to sustain stem cell self-renewal and OCT4-IB is not related to stemness. OCT4 contains a POU-specific domain (aa 138-212) that mediates its interaction with pyruvate kinase isozyme type M2 (PKM2), which regulates its transcriptional activity in as positive manner. Human Oct-4 can undergo phosphorylation by protein kinase A and/or ERK at a highly conserved serine 236 within the POU DNA-binding homeodomain. Phosphorylation at this site sterically hinders both its DNA binding and homodimer assembly. OCT4 can undergo sumoylation that enhances its stability, DNA binding and transactivation activity. WWP2, an E3 ubiquitin ligase is shown to specifically interact with OCT4 through its tryptophan-based WW domains, which promotes its ubiquitination and proteasomal degradation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lee, J., et al. (2008). Int. J. Biochem. Cell Biol. 40(5); 1043-54; Zeineddine, D., et al. (2014). Am. J. Stem Cells. 3(2);74-82).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Margarita Pustovalova et al.
International journal of molecular sciences, 23(9) (2022-05-15)
Cancer stem cells (CSCs) play a critical role in the initiation, progression and therapy relapse of many cancers including non-small cell lung cancer (NSCLC). Here, we aimed to address the question of whether the FACS-sorted CSC-like (CD44 + &CD133 +)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service