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ZRB1312

Sigma-Aldrich

Anti-β-Actin Antibody, clone 6L12, ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Synonym(s):
Actin cytoplasmic 1

biological source

rabbit (recombinant)

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

6L12, monoclonal
recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 41.74 kDa
observed mol wt ~42 kDa

species reactivity

rat, mouse, monkey, human

species reactivity (predicted by homology)

bovine, porcine

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... ACTB(60)

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ZMS1004ZMS1156ZRB1407
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

rabbit (recombinant)

biological source

mouse

biological source

mouse

biological source

rabbit

Gene Information

human ... ACTB(60)

Gene Information

human ... ACTA2(59)

Gene Information

human ... ACTB(60)

Gene Information

human ... ANLN(54443)

species reactivity

rat, mouse, monkey, human

species reactivity

mouse, rat, human

species reactivity

monkey, mouse, human, rat

species reactivity

human, mouse

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 6L12 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects beta-Actin in multiple species. It targets an epitope within 16 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human beta-Actin.

Application

Anti-beta-Actin, clone 6L12 ZooMAb, Cat. No. ZRB1312, is a recombinant rabbit monoclonal antibody that specifically detects beta-Actin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected -Actin in C2C12, C6, and COS-1 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected -Actin in human uterus and human stomach tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected -Actin in C2C12 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Actin, cytoplasmic 1 (UniProt: P60709; also known as beta-Actin) is encoded by the ACTB gene (Gene ID: 60) in human. Actins are ubiquitous eukaryotic proteins that serve as multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, transcriptional regulation, and vesicle trafficking. These functions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Beta- and gamma-actins are the major structural components of the actin cytoskeleton in non-muscle cells. Beta-actin plays a predominant role in cell migration and its enrichment has also been observed at the leading edge of migrating cells, whereas gamma-actin appears to be more uniformly distributed throughout the cell. In addition, beta-actin can also undergo N-terminal arginylation that regulates lamella formation in motile cells. Beta-actin has also been consistently utilized as a loading control in Western blotting applications due to the fact that its expression is fairly consistent, although its levels can somewhat differ in different tissues and its expression can be affected by certain pathological conditions. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Chien-Chang Kao et al.
International journal of molecular sciences, 23(4) (2022-02-27)
Urothelial carcinoma includes upper urinary tract cancer (UTUC) and bladder cancer. Although nephroureterectomy is the standard treatment for UTUC, the recurrence rate is approximately half and the tumor is associated with poor prognoses. Metastases are the most devastating and lethal
Lucy G Thorne et al.
The EMBO journal, 40(15), e107826-e107826 (2021-06-09)
SARS-CoV-2 infection causes broad-spectrum immunopathological disease, exacerbated by inflammatory co-morbidities. A better understanding of mechanisms underpinning virus-associated inflammation is required to develop effective therapeutics. Here, we discover that SARS-CoV-2 replicates rapidly in lung epithelial cells despite triggering a robust innate
Yu Huang et al.
Journal of the American Heart Association, 10(21), e021895-e021895 (2021-10-30)
Background Exploring potential therapeutic target is of great significance for myocardial infarction (MI) and post-MI heart failure. Transcription factor Yin-Yang 1 (YY1) is an essential regulator of apoptosis and angiogenesis, but its role in MI is unclear. Methods and Results
Leilei Lin et al.
Journal of translational medicine, 19(1), 123-123 (2021-03-25)
As a common haematological malignancy, acute myeloid leukaemia (AML), particularly with extramedullary infiltration (EMI), often results in a high mortality rate and poor prognosis. Circular RNAs (circRNAs) regulate biological and pathogenic processes, suggesting a potential role in AML. We have
Yanfang Gao et al.
International journal of molecular medicine, 46(4), 1582-1592 (2020-09-19)
The inhibition of mesangial cell proliferation has become an important therapy for the prevention of glomerular proliferation‑associated diseases. The combined application of immunosuppressants with multiple targets presents a novel direction in the treatment of kidney diseases. The present study was

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