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AB5803

Sigma-Aldrich

Anti-Sox4 Antibody

Chemicon®, from rabbit

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Synonym(s):
Anti-Anti-CSS10, Anti-Anti-EVI16
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SOX4(6659)

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AV38234AMAB91378AMAB91380
Sigma-Aldrich

Sigma-Aldrich

AB5803

Anti-Sox4 Antibody

antibody form

affinity purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

polyclonal

clone

polyclonal

clone

CL5634, monoclonal

clone

CL5665, monoclonal

species reactivity

mouse, human

species reactivity

rabbit, rat, guinea pig, bovine, mouse, human, dog

species reactivity

mouse, rat, human

species reactivity

human, mouse, rat

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunofluorescence: 2-10 μg/mL, immunohistochemistry: 1:1000-1:2500

technique(s)

immunofluorescence: 2-10 μg/mL, immunohistochemistry: 1:1000-1:2500

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Specificity

Recognizes Sox4. The molecular weight of the protein is 47,258 Daltons.

Immunogen

Synthetic peptide, amino acids 34-50 of Sox4.

Application

Anti-Sox4 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Sox4 & has been validated in WB, ICC.

Linkage

Replaces: AB9092

Storage and Stability

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (~10,000G) for 15 minutes to pellet the precipitated antibody product. There will be a whitish pellet near the bottom. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2


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Manami Hiraiwa et al.
Communications biology, 5(1), 22-22 (2022-01-13)
Glioma stem cells (GSCs) contribute to the pathogenesis of glioblastoma, the most malignant form of glioma. The implication and underlying mechanisms of SMAD specific E3 ubiquitin protein ligase 2 (SMURF2) on the GSC phenotypes remain unknown. We previously demonstrated that
The transcription factor Sox4 is a downstream target of signaling by the cytokine TGF-? and suppresses T(H)2 differentiation.
Kuwahara, M; Yamashita, M; Shinoda, K; Tofukuji, S; Onodera, A; Shinnakasu, R; Motohashi et al.
Nature Immunology null
Yu-Ming Yeh et al.
International journal of cancer, 133(4), 867-878 (2013-02-08)
Metastasis is the major factor affecting patient survival in ovarian cancer. However, its molecular mechanisms remain unclear. Our study used isogenic pairs of low- and high-invasive ovarian cancer cell lines to demonstrate the downregulation of miRNA-138 in the highly invasive
Lise Sofie Haug Nissen-Meyer et al.
Journal of cell science, 120(Pt 16), 2785-2795 (2007-07-27)
The transcription factor Sox4 is vital for fetal development, as Sox4(-/-) homozygotes die in utero. Sox4 mRNA is expressed in the early embryonic growth plate and is regulated by parathyroid hormone, but its function in bone modeling/remodeling is unknown. We
Hiroyuki Yoshitomi et al.
Nature communications, 9(1), 3762-3762 (2018-09-21)
In human inflammatory sites, PD-1hiCXCR5-CD4+ T cells are involved in the formation of ectopic lymphoid-like structures (ELSs) by the secretion of chemokine CXCL13, but how the transcription of CXCL13 is regulated in CD4+ T cells is still unclear. Here we

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