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AB9080

Sigma-Aldrich

Anti-Ryanodine Receptor 2 Antibody

serum, Chemicon®

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Synonym(s):
Anti-ARVC2, Anti-ARVD2, Anti-RYR-2, Anti-RyR, Anti-VACRDS, Anti-VTSIP
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human, rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... RYR2(6262)

Specificity

Ryanodine Receptor 2.

Immunogen

Synthetic peptide from the variant TM region of human Ryanodine Receptor 2.

Application

Anti-Ryanodine Receptor 2 Antibody is an antibody against Ryanodine Receptor 2 for use in IC, IH & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Signaling Neuroscience
Western blot: 1:1,000 using ECL. The antibody reacts with the 500 kDa Ryanodine Receptor 2 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.

Immunocytochemistry: 1:1,000

Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.

Optimal working dilutions must be determined by the end user.

Physical form

Rabbit serum. Liquid.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Western blot = adult mouse cardiac muscle or brain (adult mouse skeletal muscle will be negative).

IHC = adult mouse cerebellum Purkinje cells (adult mouse cerebellum granular cells will be negative).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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