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AB9610

Sigma-Aldrich

Anti-Olig-2 Antibody

Chemicon®, from rabbit

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Synonym(s):
Oligodendrocyte transcription factor 2, Oligo2, Class B basic helix-loop-helix protein 1, bHLHb1, Class E basic helix-loop-helix protein 19, bHLHe19, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human, rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... OLIG2(10215)

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AB9610

Anti-Olig-2 Antibody

vibrant-m

AB15328

Anti-Olig2 Antibody

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ABN899

Anti-Olig-2 Antibody

vibrant-m

MABN50

Anti-Olig2 Antibody, clone 211F1.1

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

species reactivity

mouse, human, rat

species reactivity

rat

species reactivity

mouse, rat, human

species reactivity

mouse

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

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General description

Oligodendrocyte transcription factor 2 (UniProt: Q13516, also known as Oligo2, Class B basic helix-loop-helix protein 1, bHLHb1, Class E basic helix-loop-helix protein 19, bHLHe19, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17) is encoded by the OLIG2 (also known as BHLHB1, BHLHE19, PRKCBP2, RACK17) gene (Gene ID: 10215) in human. Olig2 is expressed in the brain in oligodendrocytes. Its strong expression is also observed in oligodendrogliomas. Olig2 is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. It functions together with ZNF488 to promote oligodendrocyte differentiation. Olig2 is observed in both nucleus and the cytoplasm. Its nuclear localization signal is contained in the bHLH domain (aa 108-162). It can be masked in the native form and translocation to the nucleus may be mediated by interaction either with class E bHLH partner protein or with NKX2-2. Olig2 cooperates with Olig1 to establish the motor neuron progenitor domain of the embryonic neural tube. Removal of Cdx transcription factors is shown to result in the continued induction of Olig2 and ectopic production of cranial motor neuron progenitors. A chromosomal aberration involving Olig2 is shown to cause a form of T-cell acute lymphoblastic leukemia (T-ALL). (Ref.: Metzis, V., et al. (2018). Cell 175(4); 1105-1118).

Specificity

Other species have not been tested.
Recognizes the ~32 kDa Olig-2 protein by Western blot.

Immunogen

Recombinant mouse Olig-2

Application

Anti-Olig-2 Antibody is an antibody against Oligodendrocyte transcription factor 2 for use in IC, IH, IH(P), IP and WB.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Western Blot Analysis:
1:2,500-1:5,000 dilution of a previous lot was used in western blot on human, rat and mouse cell lines (human oligodendroglioma, rat primary neurepithelial, mouse transfected) and tissues (brain and spinal cord).

Immunoprecipitation:
1:500-1:1,000 with human oligodendroglioma and mouse transfected cell line.
Optimal working dilutions must be determined by the end user.

Immunocytochemistry:
1:500-1:1,000 dilution of a previous lot was used in immunocytochemistry on human, rat and mouse cell lines (human oligodendroglioma, rat primary neurepithelial, mouse transfected) and tissues (brain and spinal cord).

Optimal working dilutions must be determined by the end user

Quality

Evaluated by immunohistochemistry on glioblastoma

Immunohistochemistry (Paraffin):
Representative lot data.
Anti-Olig2 (AB9610) staining pattern morphology in glioblastoma. Tissue was pretreated with TE Buffer, pH 9.0. Polyclonal antibody was diluted to 1:500, using IHC-Select Detection with HRP-DAB.
Optimal Staining With TE Buffer Epitope Retrieval: Glioblastoma

Target description

~32 kDa

Physical form

Ammonium Sulfate Precipitation
Format: Purified
Purified rabbit polyclonal. Liquid in 100 mM Glycine, 200 mM Tris, pH 8.0.

Storage and Stability

Stable for 6 months at 2-8ºC from date of receipt.

Analysis Note

Control
Mouse brain whole cell lysate, PC12 whole cell lysate, brain (rat) tissue lysate

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Inga Nazarenko et al.
PloS one, 6(4), e18303-e18303 (2011-04-15)
Deregulation of platelet-derived growth factor (PDGF) signaling is a hallmark of malignant glioma. Two alternatively spliced PDGF-A mRNAs have been described, corresponding to a long (L) and a short (S) isoform of PDGF-A. In contrast to PDGF-A(S), the PDGF-A(L) isoform
PCP effector proteins inturned and fuzzy play nonredundant roles in the patterning but not convergent extension of mammalian neural tube.
Heydeck W, Liu A.
Developmental Dynamics null
The probable cell of origin of NF1- and PDGF-driven glioblastomas.
Hambardzumyan, D; Cheng, YK; Haeno, H; Holland, EC; Michor, F
Testing null
Loss of ATM/Chk2/p53 pathway components accelerates tumor development and contributes to radiation resistance in gliomas.
Squatrito, M; Brennan, CW; Helmy, K; Huse, JT; Petrini, JH; Holland, EC
Cancer Cell null
F Giannitti et al.
Veterinary pathology, 51(4), 832-845 (2013-09-21)
Reports of primary nervous system tumors in wild raccoons are extremely rare. Olfactory tumors were diagnosed postmortem in 9 free-ranging raccoons from 4 contiguous counties in California and 1 raccoon from Oregon within a 26-month period between 2010 and 2012.

Articles

Leverage the inherent immune ability of an animal to generate antibodies that bind to specific molecules.

Explore the basics of working with antibodies including technical information on structure, classes, and normal immunoglobulin ranges.

Uses for immunofluorescence (IF)—where an antibody conjugated to a molecule that fluoresces when excited by lasers— include protein localization, confirmation of post-translational modification or activation, and proximity to/complexing with other proteins.

Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.

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Protocols

Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

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