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MABN50

Sigma-Aldrich

Anti-Olig2 Antibody, clone 211F1.1

clone 211F1.1, from mouse

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Synonym(s):
oligodendrocyte lineage transcription factor 2, oligodendrocyte-specific bHLH transcription factor 2, human protein kinase C-binding protein RACK17, Protein kinase C-binding protein 2, protein kinase C binding protein 2, Class B basic helix-loop-helix pr
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

clone

211F1.1, monoclonal

species reactivity

mouse

species reactivity (predicted by homology)

rat (based on 100% sequence homology), human (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... OLIG2(10215)

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This Item
AB9610ABN899HPA003254
vibrant-m

MABN50

Anti-Olig2 Antibody, clone 211F1.1

vibrant-m

AB9610

Anti-Olig-2 Antibody

vibrant-m

ABN899

Anti-Olig-2 Antibody

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

rabbit

Gene Information

human ... OLIG2(10215)

Gene Information

human ... OLIG2(10215)

Gene Information

human ... OLIG2(10215)

Gene Information

human ... OLIG2(10215)

clone

211F1.1, monoclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

mouse

species reactivity

mouse, human, rat

species reactivity

mouse, rat, human

species reactivity

human

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

Oligodendrocyte lineage transcription factor 2 (Olig2) is a protein belonging to group A of the OLIG family of basic helix-loop-helix transcription factors (bHLH). bHLH proteins are divided into 6 groups (A-F), and characterized by their biochemical properties and structure. Olig2 is classified as group A due to its ability to form heterodimers with other bHLH proteins and binding to the E box. Olig2 has a crucial role during development in specifying the final location of motor neurons and oligodendrocytes in the spinal cord, along with the development within the hindbrain of somatic motor neurons. Olig2 expression is observed within oligodendrocytes and in developing astrocytes. Recent studies have shown strong expression of Olig2 in human oligodendrogliomas, primary tumors within the central nervous system, and moderate expression in astrocytomas. These observations have led to the proposal of Olig2 having potential as an oligodendroglioma diagnostic marker.

Specificity

This antibody recognizes Olig2.

Immunogen

Epitope: Unknown
Recombinant protein corresponding to human Olig2.

Application

Detect Olig2 using this Anti-Olig2 Antibody, clone 211F1.1 validated for use in WB, IC, IH & IP.
Immunohistochemistry Analysis: This antibody has been shown to detect Olig2 in coronal brain tissue as reported by an independent laboratory (Cai, J., et al. (2007). Development. 134:1887-1899).

Immunocytochemistry Analysis: This antibody has been shown to detect Olig2, as reported by an independent laboratory (Lee, S.K., et al. (1995). Genes & Dev. 19:282-294).

Immunoprecipitation Analysis: This antibody has been shown to detect Olig2, as reported by an independent laboratory (Lee, S.K., et al. (1995). Genes & Dev. 19:282-294).
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Quality

Evaluated by Western Blot in mouse brain lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected Olig2 on 10 µg of mouse brain lysate.

Target description

~37 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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An efficient and economical culture approach for the enrichment of purified oligodendrocyte progenitor cells.
Jianqin Niu,Lingyun Wang,Shubao Liu,Chengren Li,Jiming Kong,Hai-Ying Shen,Lan Xiao
Journal of Neuroscience Methods null
Contribution of microRNA-1275 to Claudin11 protein suppression via a polycomb-mediated silencing mechanism in human glioma stem-like cells.
Katsushima, K; Shinjo, K; Natsume, A; Ohka, F; Fujii, M; Osada, H; Sekido, Y; Kondo, Y
The Journal of Biological Chemistry null
Diverse behaviors of outer radial glia in developing ferret and human cortex.
Gertz, CC; Lui, JH; LaMonica, BE; Wang, X; Kriegstein, AR
The Journal of Neuroscience null
Expression of klotho mRNA and protein in rat brain parenchyma from early postnatal development into adulthood.
Clinton, SM; Glover, ME; Maltare, A; Laszczyk, AM; Mehi, SJ; Simmons, RK; King, GD
Brain Research null
Michael J E Joseph et al.
Translational stroke research, 7(3), 192-208 (2016-01-09)
Damage to myelinated axons contributes to neurological deficits after acute CNS injury, including ischemic and hemorrhagic stroke. Potential treatments to promote re-myelination will require fully differentiated oligodendrocytes, but almost nothing is known about their fate following intracerebral hemorrhage (ICH). Using

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