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MAB079-1

Sigma-Aldrich

Anti-S-100 Protein Antibody, clone 15E2E2

clone 15E2E2, Chemicon®, from mouse

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Synonym(s):
Calcium Binding Protein A1/B1
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

15E2E2, monoclonal

species reactivity

bovine, rat, human, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... S100A1(6271)

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Anti-FSP1/S100A4 Antibody

vibrant-m

MABC653

Anti-S100A4 Antibody, clone 9D5.1

species reactivity

bovine, rat, human, mouse

species reactivity

mouse, human

species reactivity

human, mouse

species reactivity

human, mouse

clone

15E2E2, monoclonal

clone

polyclonal

clone

2A5, monoclonal

clone

9D5.1, monoclonal

antibody form

purified immunoglobulin

antibody form

-

antibody form

purified antibody

antibody form

purified immunoglobulin

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

-

isotype

IgG2aκ

isotype

IgG

isotype

IgG1

isotype

IgG1κ

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General description

S-100 protein is a member of the EF-hand calcium-binding protein superfamily that is widely distributed and conserved in the central nervous system of vertebrates. The protein exists in both heterodimeric and homodimeric forms. The two subunits of S-100 protein are the products of separate genes that are differentially expressed by various cells. The beta subunit is present in all S-100 positive cells and tumors. In contrast, the alpha subunit is detectable only in heart, skeletal muscle and brain. In addition to its calcium binding properties, S-100 protein has a high-affinity binding site for zinc and is involved in the regulation of protein phosphorylation in the brain. The protein apparently plays a role in cell differentiation and growth, cytoskeletal structure and function, and has been implicated in neuropathological diseases.

Specificity

This antibody recognizes ~22 kDa (non-reduced) or 11 kDa (reduced) of S100. It reacts with human, rat, mouse, and cow (bovine). Other species are not tested.

Immunogen

Epitope: Recognizes both alpha & beta S100
Purified bovine S-100 protein

Application

Anti-S-100 Protein Antibody, clone 15E2E2 is an antibody against S-100 Protein for use in IH, IH(P), IP & WB.
Immunoprecipitation:
A previous lot of this antibody was used in IP.

Visualization of cellular and subcellular localization of S-100 protein using immunohistochemical techniques.

Immunohistochemistry:
A 1:100-200 dilution of a previous lot was used for paraffin embedded tissue sections.

Optimal working dilutions must be determined by end user.
Research Category
Metabolism
Research Sub Category
Muscle Physiology

Quality

Evaluated by Western Blot on PC12 lysates

Western Blot Analysis:
1:1000 dilution of this antibody detected S100 on 10 µg of PC12 lysate.

Physical form

Format: Purified
The mouse IgG is purified by protein A-affinity chromatography according to isotyping.
This affinity purified antibody is supplied in sterile phosphate buffered saline (pH 7.2) containing antibody stabilizer. Contains 0.01% sodium azide.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
Positive Control Tissue: Melanoma

Other Notes

Concentration: 0.5 mg/mL

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Cellular immunolocalization of S100 protein within fixed tissue sections by monoclonal antibodies.
Loeffel, S C, et al.
Archives of Pathology & Laboratory Medicine, 109, 117-122 (1985)
Daria Guseva et al.
Brain : a journal of neurology, 132(Pt 8), 2180-2195 (2009-06-23)
The adhesion molecule L1 is one of the few adhesion molecules known to be beneficial for repair processes in the adult central nervous system of vertebrates by promoting axonal growth and neuronal survival. In the peripheral nervous system, L1 is
RAGE-TXNIP axis is required for S100B-promoted Schwann cell migration, fibronectin expression and cytokine secretion.
Sbai, O; Devi, TS; Melone, MA; Feron, F; Khrestchatisky, M; Singh, LP; Perrone, L
Journal of Cell Science null
D W Zochodne et al.
Journal of anatomy, 196 ( Pt 2), 279-283 (2000-03-30)
Classic ideas on mechanisms for axon sprouting and nerve regeneration from peripheral nerves suggest that there is a prominent role for neurotrophin support. There has been comparatively less attention towards features of the regenerative process that develop from the proximal
Elad Maor et al.
Journal of vascular and interventional radiology : JVIR, 21(11), 1708-1715 (2010-10-12)
To evaluate the effect of endovascular nonthermal irreversible electroporation (NTIRE) on blood vessels. Specially made endovascular devices with four electrodes on top of inflatable balloons were used to apply electroporation pulses. Finite element simulations were used to characterize NTIRE protocols

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