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L4543

Sigma-Aldrich

Anti-L1CAM antibody, Mouse monoclonal

clone UJ127.11, purified from hybridoma cell culture

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Synonym(s):
L1CAM Antibody - Anti-L1CAM antibody, Mouse monoclonal, L1Cam Antibody, Anti-CAML1, Anti-L1 Cell Adhesion Molecule, Anti-MIC5, Anti-Neural Cell Adhesion Molecule L1
MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

UJ127.11, monoclonal

form

buffered aqueous solution

mol wt

antigen 220-240 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using cell extracts of G361 cells (human melanoma)

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... L1CAM(3897)

General description

Anti-L1CAM antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma UJ127.11 produced by the fusion of mouse myeloma cells (P3- X63-Ag8-653 cells) and splenocytes from mice immunized with human fetal brain. L1 cell adhesion molecule (L1CAM) protein belongs to the family of cell adhesion molecules (CAM).
The gene L1CAM (L1 cell adhesion molecule) encodes a member of the immunoglobulin supergene family. The encoded protein contains a cytoplasmic intracellular domain, one single-pass transmembrane region and an extracellular domain containing six immunoglobulin- (Ig) and five fibronectin (Fn) III-like motifs. The gene is mapped to human chromosome Xq28. It contains 29 exons that encode a protein of 1257 amino acids containing a 19 amino acid signal peptide and a functional L1 protein of 1238 amino acids. It is mainly expressed in the nervous system.

Immunogen

L1CAM from human fetal brain.

Application

Anti-L1CAM antibody has been used in immunohistochemistry.
Anti-L1CAM antibody, Mouse monoclonal has been used for:
  • immunohistochemistry
  • immunoprecipitation
  • immunoblotting
  • immunocytochemistry
  • flow cytometry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

L1 cell adhesion molecule (L1CAM) is critical for central nervous system (CNS) development in humans. This protein is responsible for many activities among them: neurite outgrowth, adhesion, fasciculation and myelination. These activities are mediated by the interaction of L1CAM with various members of the CAM protein family, extracellular matrix molecules and signaling receptors, through interactions that involve the extracellular domain of L1CAM. Mutations in the L1CAM gene may be linked to several brain disorders such as type 1 X-linked spastic paraplegia (SPG1), MASA syndrome and ACC (agenesis of corpus callosum).
The gene L1CAM (L1 cell adhesion molecule) encodes a glycoprotein that functions in axon growth during development and axon bundling. It interacts with Schwann cells and axons, and mediates neuronal cell migration, and neuronal cell survival. Overexpression of this protein is associated with tumor progression. It has been found to mediate therapeutic resistance in ovarian and pancreatic cancer cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Yasuhiro Ikegami et al.
Frontiers in bioengineering and biotechnology, 12, 1259138-1259138 (2024-02-13)
Motor nerve organoids could be generated by culturing a spheroid of motor neurons differentiated from human induced pluripotent stem (iPS) cells within a polydimethylsiloxane (PDMS) chip which guides direction and fasciculation of axons extended from the spheroid. To isolate axon
L1 cell adhesion molecule (L1CAM) in invasive tumors
Raveh S, et al.
Cancer letters, 282(2), 137-145 (2009)
Genetic and clinical aspects of X-linked hydrocephalus (L1 disease): mutations in the L1CAM gene
Weller S and Gartner J
Human Mutation, 18(1), 1-12 (2001)
Meredith Stevers et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 32(1), 88-99 (2018-09-02)
Well-differentiated papillary mesothelioma is an uncommon mesothelial neoplasm that most frequently arises in the peritoneal cavity of women of reproductive age. Whereas malignant mesothelioma is an aggressive tumor associated with poor outcome, well-differentiated papillary mesothelioma typically exhibits indolent behavior. However
Dario Van Meenen et al.
International journal of molecular sciences, 23(20) (2022-10-28)
A disintegrin and metalloproteinase (ADAM) family proteins, acting as sheddases, are important factors in a number of pathologies, including cancer, and have been suggested as promising therapeutic targets. The study presented focuses on the involvement of ADAM10 and ADAM17 in

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