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ZMS1019

Sigma-Aldrich

Anti-Olig2 Antibody, clone 211F1.1 ZooMAb® Mouse Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Synonym(s):
Class B basic helix-loop-helix protein 1, Class E basic helix-loop-helix protein 19, Oligo2, Oligodendrocyte transcription factor 2, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17, bHLHb1, bHLHe19

biological source

mouse (recombinant)

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

211F1.1, recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 32.38 kDa
observed mol wt ~37 kDa

species reactivity

rat, human, mouse

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG2a

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... OLIG2(10215)
mouse ... Olig2(50913)
rat ... Olig2(304103)

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antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

clone

211F1.1, recombinant monoclonal

clone

recombinant monoclonal

clone

SKB1, recombinant monoclonal

clone

1J12, recombinant monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

species reactivity

rat, human, mouse

species reactivity

rat, mouse

species reactivity

rat, human, mouse

species reactivity

mouse, human

Gene Information

human ... OLIG2(10215)
mouse ... Olig2(50913)
rat ... Olig2(304103)

Gene Information

human ... OLIG2(10215)
mouse ... Olig2(50913)
rat ... Olig2(304103)

Gene Information

human ... AKT1(207)

Gene Information

human ... ARNTL(406)

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 211F1.1 is a ZooMAb mouse recombinant monoclonal antibody that specifically detects Oligodendrocyte transcription factor 2 (Olig2).

Immunogen

Recombinant human Oligodendrocyte transcription factor 2 (Olig2).

Application

Anti-Olig2, clone 211F1.1 ZooMAb, Cat. No. ZMS1019, is a recombinant Mouse monoclonal antibody that targets Oligodendrocyte transcription factor 2 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Olig2 in rat brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Olig2 in human cerebral cortex tissue sections.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Olig2 in Oligodendrocyte precursor cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Oligodendrocyte transcription factor 2 (UniProt: Q13516; also known as Oligo2, Class B basic helix-loop-helix protein 1, bHLHb1, Class E basic helix-loop-helix protein 19, bHLHe19, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17, Olig2) is encoded by the OLIG2 (also known as BHLHB1, BHLHE19, PRKCBP2, RACK17) gene (Gene ID: 10215) in human. Olig2 is a basic helix-loop-helix (bHLH) transcription factor that is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. It is expressed in oligodendrocytes and stronger expression is reported in oligodendrogliomas. It functions together with ZNF488 to promote oligodendrocyte differentiation and cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. A chromosomal aberration involving OLIG2 is reported to cause a form of T-cell acute lymphoblastic leukemia (T-ALL). OLIG2 expression is induced by sonic hedgehog and fibroblast growth factor during development. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Related Content

Choose sustainable options for your research with greener alternative ZooMAb® recombinant antibodies. These BSA-free, carrier-free antibodies are shipped in low-waste packaging and offer maximum stability without compromising performance.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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