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ABC929

Sigma-Aldrich

Anti-LC3-I/II Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 light chain 3 alpha
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

Xenopus (based on 100% sequence homology), rat (based on 100% sequence homology), porcine (based on 100% sequence homology), mouse (based on 100% sequence homology), zebrafish (based on 100% sequence homology), bovine (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

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This Item
ABC232AB15412SAB1306617
Anti-LC3-I/II Antibody from rabbit, purified by affinity chromatography

ABC929

Anti-LC3-I/II Antibody

Anti-LC3 Antibody serum, from rabbit

ABC232

Anti-LC3 Antibody

Sigma-Aldrich

AB15412

Anti-MAP1LC3 A Antibody

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunofluorescence: 1:25, immunohistochemistry: 1:10-1:50, western blot: 1:1000

UniProt accession no.

Q9H492

UniProt accession no.

Q9H492

UniProt accession no.

Q9H492

UniProt accession no.

Q9H492

species reactivity

human

species reactivity

human, rat, mouse

species reactivity

rat, human

species reactivity

human, mouse

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General description

Microtubule-associated LC3A constitute nearly half of the mass of all the microtubule associated proteins that copurify with brain microtubules. LC3A is composed of a heavy chain and multiple light-chain subunits. The molecular machinery required for autophagy is highly conserved in all eukaryotes as seen by the high degree of conservation of proteins involved in the formation of the autophagosome membranes. Recently, both yeast Apg8p and its rat homologue LC3-II were identified as essential constituents of autophagosome membrane as a processed form. In addition, both the yeast and human proteins exist in two modified forms produced by a series of post-translational modifications including a critical C-terminal cleavage after a conserved Gly residue, and the smaller processed form is associated with the autophagosome membranes Results revealed different regulation of the three human isoforms of LC3-II and implicate that the three isoforms may have different physiological functions. Two forms of LC3A, called LC3-I and -II, were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes.

Specificity

This antibody detects both the non-lipidated LC3-I (non-autophagic) and lipidated LC3-II (autophagic) under autophagy conditions.

Immunogen

Recombinant protein corresponding to Human LC3-I/II.

Application

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected LC3 in serum-starved, chloroquine-treated HeLa cells. A punctate distribution is seen, evidence of autophagy conditions within the cell.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-LC3-I/II Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of LC3-I/II.

Quality

Evaluated by Western Blotting in serum-starved, chloroquine-treated HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected LC3-I (non-lipidated) and LC3-II (lipidated) in 10 µg of serum-starved, chloroquine-treated HeLa cell lysate.

Target description

~17/14 kDa observed

Linkage

Replaces: MABC177; MABC176

Physical form

Antigen Affinity Purified
Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Autophagy is a highly regulated process that is involved in cell growth, development, and death. In autophagy cells destroy their own cytoplasmic components in a very systematic manner and recycle them.

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