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L7543

Sigma-Aldrich

Anti-LC3B antibody produced in rabbit

enhanced validation

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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Synonym(s):
LC3B Antibody - Anti-LC3B antibody produced in rabbit, Lc3 Antibody Sigma, Lc3B Antibody, Anti-Autophagy-related protein LC3 B, Anti-Autophagy-related ubiquitin-like modifier LC3 B, Anti-MAP1 light chain 3-like protein 2, Anti-MAP1A/1B light chain 3 B, Anti-MAP1A/MAP1B LC3 B, Anti-Microtubule-associated proteins 1A/B light chain 3B precursor
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 16-18 kDa

species reactivity

mouse, human, rat

enhanced validation

functional assay
Learn more about Antibody Enhanced Validation

concentration

~1 mg/mL

technique(s)

indirect immunofluorescence: 5-10 μg/mL using human HeLa cells
western blot: 1-2 μg/mL using whole extracts of rat and mouse brain

UniProt accession no.

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

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This Item
L8918SAB4200361ABC929
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

LC3B-6, monoclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

-

mol wt

antigen 16-18 kDa

mol wt

antigen 16-18 kDa

mol wt

antigen ~18/16 kDa

mol wt

-

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General description

Microtubule-associated protein 1 light chain 3 (MAPLC3/LC3) was first identified in rat as a protein that co-purifies with microtubule-associated protein 1A and 1B from rat brain. LC3 exists in cells in two forms. One is cytoplasmic, LC3-I (18 kDa) and the other, LC3-II (16 kDa) is associated with the autophagosome membrane. LC3-I is modified to a membrane-bound form, LC3-II (a LC3-phospholipid conjugate), by mammalian autophagy related protein 7 (Atg7) and Atg3, which are E1- and E2-like enzymes, respectively. Three human orthologs of the rat LC3, named MAP1LC3A/LC3A, MAP1LC3B/LC3B, and MAP1LC3C/ LC3C, were identified. The human proteins exhibit two forms representing the cytosolic (type I) and the membrane associated (type II) forms. The three human isoforms show different expression patterns in human tissues.
The gene MAP1LC3B (microtubule associated protein 1 light chain 3 β) is a homolog of ATG8 gene, an autophagy-related gene in yeast. The encoded protein is called pro-MAP1LC3B, a pro-form that is cleaved by ATG4 to produce a cytosolic form called MAP1LC3B-I.

Specificity

By immunoblotting, the antibody recognizes human, rat, and mouse LC3B-I and LC3B-II (~18 kDa and ~16 kDa, respectively).

Immunogen

synthetic peptide corresponding to amino acids 2-15 of human LC3B, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence differs by one amino acid in rat and mouse.

Application

Anti-LC3B antibody produced in rabbit has been used in western blotting and immunohistochemistry.
Anti-LC3B antibody produced in rabbit has been used in western blotting and immunohistochemistry.

Biochem/physiol Actions

MAP1LC3B-I (microtubule associated protein 1 light chain 3 β-I) conjugates with MAP1LC3B-II before being inserted into both inner and outer membranes of the growing autophagic vesicle during autophagy. The cellular distribution and processing of MAP1LC3B facilitates monitoring of autophagy. Cells that lack MAP1LC3B are found to be defective in the formation of autophagic vesicles, with altered phenotypes associated with the role of autophagy in energy homeostasis and removal of protein aggregates. It can serve as an autophagic marker.
Microtubule-associated protein 1 light chain 3 (MAPLC3/LC3) expression level correlates with the extent of autophagosome formation. LC3B can be used as an autophagosomal marker.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Yehui Duan et al.
Food & function, 10(8), 5152-5165 (2019-08-03)
The aim of this study was to investigate the effects of dietary β-hydroxy-β-methylbutyrate (HMB) on lipopolysaccharide (LPS)-induced muscle atrophy and to investigate the mechanisms involved. Sixty pigs (21 ± 2 days old, 5.86 ± 0.18 kg body weight) were used
mRNA and protein dataset of autophagy markers (LC3 and p62) in several cell lines
Gomez-Sanchez R
Data in Brief, 7(7), 641-647 (2016)
Autophagy enhances the aggressiveness of human colorectal cancer cells and their ability to adapt to apoptotic stimulus
Zheng HY, et al.
Cancer Biology & Medicine, 9(2), 105-105 (2012)
Quantitation of "autophagic flux" in mature skeletal muscle
Ju JS, et al.
Autophagy, 6(7), 929-935 (2010)
LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
Kabeya Y, et al.
The Embo Journal, 19(21), 5720-5728 (2000)

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