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P0067

Sigma-Aldrich

Anti-p62/SQSTM1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-Sequestosome 1, Anti-Ubiquitin-binding p62
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~62 kDa

species reactivity

human, rat, mouse

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): 1-2 μg using lysate of NIH-3T3 cells
indirect immunofluorescence: 1-2 μg/mL using human A549 cells
western blot: 1-2 μg/mL using whole extracts of rat PC12 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SQSTM1(8878)
mouse ... Sqstm1(18412)
rat ... Sqstm1(113894)

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This Item
P0068WH0008878M1SAB5700054
biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

rabbit

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

clone

polyclonal

clone

polyclonal

clone

2C11, monoclonal

clone

polyclonal

UniProt accession no.

Q13501

UniProt accession no.

Q13501

UniProt accession no.

Q13501

UniProt accession no.

Q13501

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General description

p62 or SQSTM1 (sequestosome 1) is a multifunctional, multi-domain adaptor protein which resides at the autophagosome membranes. The human p62 gene is mapped to human chromosome 5. The protein has 440 amino acids. This protein is conserved across metazoans, but not plant and fungi. It is composed of Phox1 and Bem1p (PB1) domain, a zinc finger (ZZ) domain, two nuclear localization signals, a tumor necrosis factor receptor-associated factor 6 (TRAF6) binding domain, a nuclear export signal, an LC3-interacting region (LIR), a Keap1-interacting region (KIR) and a ubiquitin-associated (UBA) domain. It is predominantly present in cytoplasm, but also shows expression in nucleus, autophagosomes and lysosomes.
Anti-p62/SQSTM1 is produced in rabbit using as immunogen a synthetic peptide corresponding to amino acids of human p62/SQSTM1 (GeneID: 8878), conjugated to KLH. The corresponding sequence is identical in rat and mouse. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Specificity

Anti-p62/SQSTM1 recognizes human, rat, and mouse p62/SQSTM1. The antibody may be used in various immunochemical techniques including immunoblotting (~ 62 kDa), immunoprecipitation, and indirect immunofluorescence. Detection of the p62/SQSTM1 band by immunoblotting is specifically inhibited by the immunizing peptide.

Immunogen

Synthetic peptide corresponding to amino acids of human p62/SQSTM1, conjugated to KLH. The corresponding protein sequence is identical in rat and mouse.

Application

Anti-p62/SQSTM1 antibody produced in rabbit has been used in:
  • western blotting
  • immunoprecipitation in human cell lines
  • Immunohistochemistry prostatectomy specimens

Biochem/physiol Actions

p62 or SQSTM1 (sequestosome 1) plays a role in the catabolic metabolism of molecules involved in NF-κB (nuclear factor), mTOR (mammalian target of rapamycin), MAPK (mitogen activated protein kinase), and therefore, is involved in cell cycle and apoptosis. It is thought to function as an oncogene, and its accumulation has been associated with poor prognosis. It is accumulated in cell with defective autophagy, and its cytosolic accumulation is linked with poor prognosis in prostate cancer. It is responsible for the activation of mammalian target of rapamycin complex 1 (mTORC1) by functioning as an adaptor for mTORC1 lysosomal membrane. During Dengue virus (DENV) infection, the expression of p62 protein is reduced due to proteasomal degradation, and stable expression of p62 results in decreased DENV replication. p62 is an autophagy marker, and its accumulation is linked with aberration in autophagic degradation or inhibition of autophagy.
Mutations in this gene result in sporadic and familial Paget disease of bone. p62 is commonly found in inclusion bodies containing polyubiquitinated protein aggregates, that accumulate in several degenerative diseases. Autophagy is involved in cellular clearance of these protein aggregates. Autophagy plays an essential role in cellular differentiation, cell death, and aging. Defective autophagy may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders, and pathogen infections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Dimerisation of the UBA domain of p62 inhibits ubiquitin binding and regulates NF-κB signalling.
Long J, et al.
Journal of Molecular Biology, 396(1), 178-194 (2010)
p62/SQSTM1 functions as a signaling hub and an autophagy adaptor.
Katsuragi Y et al
FEBS Journal, 282(24), 4672-4678 (2015)
a-Synuclein controls mitochondrial calcium homeostasis by enhancing endoplasmic reticulum-mitochondria interactions.
Cali T et al
The Journal of Biological Chemistry, 287(22), 17914-17929 (2012)
Cytoplasmic accumulation of sequestosome 1 (p62) is a predictor of biochemical recurrence, rapid tumor cell proliferation, and genomic instability in prostate cancer.
Burdelski C, et al.
Clinical Cancer Research (2015)
p62/SQSTM1: A potential molecular target for treatment of atherosclerosis.
Liang XF and Guan XR
Frontiers in laboratory medicine, 1(2), 104-106 (2017)

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