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B6061

Sigma-Aldrich

Anti-Beclin 1 (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Synonym(s):
Anti-ATG6, Anti-BECN1, Anti-Coiled-coil myosin-like BCL2-interacting protein, Anti-GT197, Anti-VPS30
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~60 kDa

species reactivity

human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 using biotin/ExtrAvidin®-Peroxidase staining of heat-retrieved sections of human cerebellum
immunoprecipitation (IP): 1-2 μL using extract of human HEK-293T cells expressing recombinant human Beclin 1 fusion protein
indirect immunofluorescence: suitable
western blot: 1:250-1:500 using whole extract of human HeLa cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... BECN1(8678)

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This Item
SAB1306484SAB1306537SAB1305637
Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

conjugate

unconjugated

conjugate

-

conjugate

-

conjugate

-

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

mol wt

antigen ~60 kDa

mol wt

51896 Da

mol wt

51896 Da

mol wt

51896 Da

Gene Information

human ... BECN1(8678)

Gene Information

human ... BECN1(8678)

Gene Information

human ... BECN1(8678)

Gene Information

human ... BECN1(8678)

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General description

Beclin 1 (BECN1) or GT197 is a tumor suppressor gene. It is a Bcl-2 (B-cell lymphoma 2) interacting protein and is the mammalian ortholog of yeast Atg6/Vps30. It is a 450 amino-acid protein having three domains. The gene encoding BECN1 is localized on human chromosome 17q21.

Immunogen

synthetic peptide corresponding to amino acids 29-46 of human Beclin 1, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Application

Anti-Beclin 1 (N-terminal) antibody produced in rabbit has been used in:
  • immunofluorescent staining
  • immunoblotting
  • immunohistochemistry
  • immunoprecipitation

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Biochem/physiol Actions

Beclin 1 (BECN1) is able to complement the autophagy deficiency of yeast lacking autophagy-related gene 6 (Atg6) and restores autophagy in human MCF7 breast carcinoma cells. Beclin 1 plays a major role in the early development of embryo. It also serves as a haplo-insufficient tumor-suppressor gene. Heterozygous Beclin 1 mutant mice have increased spontaneous tumorigenesis. Beclin 1 is monoallelically deleted in a high percentage of sporadic human breast, ovarian and prostate carcinomas and is expressed at reduced levels in those tumors. In human brains BECN1 decreases in an age-dependent fashion, possibly leading to a reduction of autophagic activity during aging, which may contribute to the accumulation of mutant Huntingtin and the age-delayed disease onset of Huntington disease.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Customers Also Viewed

Regulation of Intracellular Accumulation of Mutant Huntingtin by Beclin 1
Shibata M, et al.
The Journal of biological chemistry, 281(20), 14474-14485 (2006)
The BECN1 N-terminal domain is intrinsically disordered
Lee EF, et al.
Autophagy, 3-3 (2016)
Proper autophagy is indispensable for angiogenesis during chick embryo development
Lu W, et al.
Cell Cycle, 15(13), 1742-1754 (2016)
Beclin 1, an autophagy gene essential for early embryonic development, is a haploinsufficient tumor suppressor
Yue Z, et al.
Proceedings of the National Academy of Sciences of the USA, 100(25), 15077-15082 (2003)
bis-Dehydroxy-Curcumin triggers mitochondrial-associated cell death in human colon cancer cells through ER-stress induced autophagy
Basile V, et al.
Testing, 8(1), e53664-e53664 (2013)

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