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ABD29

Sigma-Aldrich

Anti-Twist1 (Twist-related protein 1) Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Twist-related protein 1, Class A basic helix-loop-helix protein 38, bHLHa38, H-twist
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rhesus macaque, mouse, horse

species reactivity (predicted by homology)

canine (based on 100% sequence homology), pig (based on 100% sequence homology), equine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology), gorilla (based on 100% sequence homology)

packaging

antibody small pack of 25 μg

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... TWIST1(7291)

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This Item
WH0007291M1SAB1404499SAB1409773
antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

polyclonal

clone

3E11, monoclonal

clone

2F8, monoclonal

clone

1G2, monoclonal

species reactivity

human, rhesus macaque, mouse, horse

species reactivity

human

species reactivity

human

species reactivity

human

packaging

antibody small pack of 25 μg

packaging

-

packaging

-

packaging

-

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin)

technique(s)

immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL

technique(s)

capture ELISA: suitable, indirect ELISA: suitable, indirect immunofluorescence: suitable, western blot: 1-5 μg/mL

technique(s)

indirect ELISA: suitable

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General description

Twist1 is the basic helix-loop-helix transcription factor that regulates embryonic progenitor and transformed tumor cell proliferation, migration and differentiation and is also important in limb development. It has been shown that twist1 expression is high in patients suffering from rheumatic inflammatory disorders with chronically activated T helper lymphocytes and may be used as a biomarker for chronic t-cell activation. Mutations in twist1 are often associated with craniofacial and limb abnormalities in humans with Saethre-Chotzen syndrome. Twist1 dimer affinity can modify the DNA binding affinity. Twist1 has also been implicated as a promoter of tumor growth, invasion and anti-cancer-drug resistance.

Specificity

Other homologies: Rat (92% sequence homology).
This antibody recognizes Twist1 near the N-terminus.

Immunogen

Epitope: Near N-terminus
KLH-conjugated linear peptide corresponding to human Twist1 near the N-terminus.

Application

Detect Twist1 (Twist-related protein 1) using this Anti-Twist1 (Twist-related protein 1) Antibody validated for use in IH(P) & IC.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Twist1 in NIH/3T3, A431, and HeLa cells.
Research Category
Stem Cell Research
Research Sub Category
Transcription Factors

Quality

Evaluated by Immunohistochemistry in ductal breast fibroadenoma and stratified squamous epithelium and cartilage of human trachea tissues.

Immunohistochemistry Analysis: 1:500 dilution of this antibody detected Twist1 in ductal breast fibroadenoma and stratified squamous epithelium and cartilage of human trachea tissues.

Target description

21 kDa calculated

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Ductal breast fibroadenoma and stratified squamous epithelium and cartilage of human trachea tissues

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Xingbo Xu et al.
Circulation research, 116(5), 857-866 (2015-01-15)
Endocardial fibroelastosis (EFE) is a unique form of fibrosis, which forms a de novo subendocardial tissue layer encapsulating the myocardium and stunting its growth, and which is typically associated with congenital heart diseases of heterogeneous origin, such as hypoplastic left
Maria-Vittoria Sepporta et al.
Communications biology, 5(1), 42-42 (2022-01-14)
The embryonic transcription factors TWIST1/2 are frequently overexpressed in cancer, acting as multifunctional oncogenes. Here we investigate their role in neuroblastoma (NB), a heterogeneous childhood malignancy ranging from spontaneous regression to dismal outcomes despite multimodal therapy. We first reveal the
Xiaoyin Lu et al.
Placenta, 39, 45-54 (2016-03-20)
The multinucleated syncytiotrophoblast (STB) is maintained and regenerated by the fusion of underlying cytotrophoblast cells (CTBs) and is responsible for a number of functions in the human placenta. Deficiencies in this structure may result in pregnancy-associated diseases. However, the detailed
Aishah Nasir et al.
Neuro-oncology advances, 3(1), vdab030-vdab030 (2021-05-06)
Therapeutic intervention in metastatic medulloblastoma is dependent on elucidating the underlying metastatic mechanism. We investigated whether an epithelial-mesenchymal transition (EMT)-like pathway could drive medulloblastoma metastasis. A 3D Basement Membrane Extract (3D-BME) model was used to investigate medulloblastoma cell migration. Cell
Jianjian Zhu et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(14), 3820-3825 (2016-03-24)
The transcription factor Brachyury (T) gene is expressed throughout primary mesoderm (primitive streak and notochord) during early embryonic development and has been strongly implicated in the genesis of chordoma, a sarcoma of notochord cell origin. Additionally, T expression has been

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