ABE365

Anti-LSD1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse, rat
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_055828
• UniProt accession no.: O60341
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KDM1A(23028)

Description

General description

LSD1 (lysine specific demethylase 1), also known as BHC110, is a histone demethylase that specifically demethylates Lys4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted Lys4 of histone H3, and may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 Lys4 on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. LSD1 may also demethylate Lys9 of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.

Specificity

This antibody recognizes LSD1.

Immunogen

Histidine-tagged recombinant protein corresponding to full length LSD1.

Application

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated LSD1 in 0.5 mg of HeLa nuclear extract.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-LSD1 Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect LSD1 also known as Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110.

Quality

Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 0.05 µg/mL of this antibody detected LSD1 in 10 µg of HeLa nuclear extract.

Target description

~110 kDa observed. Uniprot describes two isoforms at ~92 kDa and ~95 kDa due to alternative splicing.

Linkage

Replaces: MABE545

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa nuclear extract

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable