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ABF17-I

Sigma-Aldrich

Anti-TGF-beta Receptor Antibody, type I

from rabbit, purified by affinity chromatography

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Synonym(s):
EC:2.7.11.30, TGFR-1, Activin A receptor type II-like protein kinase of 53kD, Activin receptor-like kinase 5, ALK-5, Serine/threonine-protein kinase receptor R4, SKR4, Transforming growth factor-beta receptor type I, TbetaR-I
eCl@ss:
32160702

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

species reactivity (predicted by homology)

rat, porcine, bovine

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... TGFBR1(7046)

Related Categories

General description

TGF-beta receptor type-1 (UniProt: P36897; also known as EC:2.7.11.30, TGFR-1, Activin A receptor type II-like protein kinase of 53kD, Activin receptor-like kinase 5, ALK-5, Serine/threonine-protein kinase receptor R4, SKR4, Transforming growth factor-beta receptor type I, TbetaR-I) is encoded by the TGFBR1 (also known as ALK5, SKR4) gene (Gene ID: 7046) in human. TGF-beta R1 is found in all tissues examined and is most abundant in placenta and least abundant in brain and heart. It is also expressed in a variety of cancer cell lines. TGF-beta signaling involves its binding to the TGF-beta receptor type II (TGF-beta RII), which allows it to recruit TGF-beta receptor type I (TGF-beta RI) and assemble it as a heterodimeric receptor complex. TGF-beta RII phosphorylates TGF-beta RI in the glycine-serine (GS) rich region and activates the serine/threonine kinase activity of TGF-beta RI, which in turn phosphorylates receptor-linked Smad (Small mothers against decapentaplegic) proteins. To prevent any spontaneous phosphorylation of Smads, the inhibitor molecule FKBP12 binds to the GS region of the TGF-beta RI and blocks the access of TGF-beta RII to this domain. This inhibitory effect is removed upon TGF-beta binding to the receptor.

Specificity

This polyclonal antibody specifically detects TGF-beta receptor type 1 in multiple species. It targets an epitope of 15 amino acids in the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human TGF-beta receptor 1.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected TGF-beta receptor type-1 in human spleen, human cerebellum, human kidney, human pancreas, human placenta (chorionic villi), and human placenta (decidual cells).

Flow Cytometry Analysis: 1 µg from a representative lot detected TGF-beta receptor type-1 in one million mouse splenocyte cells.
This rabbit polyclonal Anti-TGF-beta Receptor, type I, Cat. No. ABF17-I is tested in Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting for the detection of TGF-beta receptor type-1.

Quality

Evaluated by Western Blotting in rat liver tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TGF-beta receptor type-1 in 10 µg of rat liver tissue lysate.

Target description

~56 kDa observed, 55.96 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: ABF17

Other Notes

Concentration: Please refer to lot specific datasheet.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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