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ABN1018

Sigma-Aldrich

Anti-LRP1 Antibody, Intracellular domain

serum, from rabbit

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Synonym(s):
Prolow-density lipoprotein receptor-related protein 1, A2MR, Alpha-2-macroglobulin receptor, APOER, Apolipoprotein E receptor, CD91, LRP-1, LRP-85, LRPICD
eCl@ss:
32160702

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse

species reactivity (predicted by homology)

human (based on 100% sequence homology), rat (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... LRP1(4035)

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This Item
SAB2101380L2420L2295
antibody form

serum

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

rabbit

Gene Information

human ... LRP1(4035)

Gene Information

human ... LRP1(4035)

Gene Information

human ... LRP1(4035)
mouse ... Lrp1(16971), Lrp1(299858)

Gene Information

human ... LRP1(4035)
mouse ... Lrp1(16971)
rat ... Lrp1(299858)

clone

polyclonal

clone

polyclonal

clone

LRP1-11, monoclonal

clone

polyclonal

UniProt accession no.

Q07954

UniProt accession no.

Q07954

UniProt accession no.

Q07954

UniProt accession no.

Q07954

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General description

Prolow-density lipoprotein receptor-related protein 1 (UniProt Q07954; also known as A2MR, Alpha-2-macroglobulin receptor, APOER, Apolipoprotein E receptor, CD91, LRP-1) is encoded by the LRP1 (also known as A2MR, APR) gene (Gene ID 4035) in human. LRP1 is an LDLR family member expressed by vascular endothelial cells forming the blood-brain barrier (BBB), vascular mural cells (pericytes and vascular smooth muscle cells (VSMCs)), neurons, and astrocytes. LRP1 recognizes and mediates the endocytosis of more than 40 different ligands, including apolipoprotein E (apoE), APP and Aβ. LRP1 is the primary receptor mediating transport of Aβ across the BBB into circulation, thereby clearing it from the brain. LRP1 is synthesized with a signal peptide sequence (a.a. 1-19), posttranslational cleavage yields a soluble form (sLRP1) and a cell surface-bound form composed of an 515 kDa extracellular α-chain (LRP-515) and a 85 kDa single-transmembrane (a.a. 4420-4444) β-chain (LRP-85) that contains the 100-amino intracellular domain (LRPICD; a.a. 4445-4544). BACE-1, ADAM-10, ADAM-12, ADAM-17, MT1-MMP, and t-PA are shown to be involved in the shedding of LRP1 to produce sLRP1.

Specificity

Recognizes the Pro- and mature LRP-1 (a.a. 1-4544 and 20-4544, respectively), the beta-secretase-cleaved, membrane-bound LRP-85, and the gamma-secretase-cleaved intracellular domain LRPICD fragment, but not the beta-secretase-cleaved extracellular LRP-515 fragment.

Immunogen

Epitope: LRPICD.
GST-tagged recombinant human LRP1 intracellular domain (LRPICD).

Application

Anti-LRP1 Antibody, Intracellular domain is an antibody against LRP1 for use in Western Blotting.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western Blotting Analysis: A representative lot detected the LRP-85 target band in NIH/3T3 cell lysate, as well as in mouse brain and liver homogenates (Courtesy of Professor Nicola Zambrano, University of Naples Federico II, Italy).
Western Blotting Analysis: A representative lot detected a decreased LRP-85 target band in lysates from LRP1 shRNA-treated NIH/3T3 cells (Courtesy of Professor Nicola Zambrano, University of Naples Federico II, Italy).

Quality

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected the LRP-85 target band in 10 µg of NIH/3T3 cell lysate.ysate.

Target description

~80 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Rabbit polyclonal antibody serum with 0.05% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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