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L2295

Sigma-Aldrich

Anti-LRP1 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-A2MR, Anti-APOER, Anti-APR, Anti-Alpha-2-Macroglobulin receptor, Anti-Apolipoprotein E receptor, Anti-CD91, Anti-LRP, Anti-Low density lipoprotein receptor-related protein 1
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~500 kDa

species reactivity

human, mouse, rat

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 2-5 μg/mL using rat NRK cells
western blot: 1-2 μg/mL using whole extract of human U87 cells
western blot: 2-4 μg/mL using whole extract of mouse C2C12 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... LRP1(4035)
mouse ... Lrp1(16971)
rat ... Lrp1(299858)

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This Item
L2420SAB2101380WH0007804M1
biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

mouse

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

technique(s)

indirect immunofluorescence: 2-5 μg/mL using rat NRK cells, western blot: 2-4 μg/mL using whole extract of mouse C2C12 cells, western blot: 1-2 μg/mL using whole extract of human U87 cells

technique(s)

indirect immunofluorescence: suitable, western blot: 1-2 μg/mL

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

indirect ELISA: suitable, western blot: 1-5 μg/mL

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

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General description

LDL receptor-related protein 1 (LRP1), also known as 2-macroglobulin receptor, is a large endocytic receptor, widely expressed on the surface and intracellular membranes of various cells. LRP1 is encoded by the gene mapped to human chromosome 12q13.3. The encoded protein is a member of the LDL receptor family. LRP1 is synthesized in the endoplasmic reticulum as a single-chain 600 kDa type I transmembrane glycosylated precursor that is cleaved subsequently in the Golgi complex into two subunits of 515 kDa and 85 kDa. The 515 kDa N-terminal subunit remains attached to the membrane through noncovalent association with the smaller 85 kDa C-terminal subunit, which contains the transmembrane and cytoplasmic domains.

Specificity

Anti-LRP1 (N-terminal) recognizes human, mouse, and rat LRP1 (not tested in other species).

Application

Anti-LRP1 (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

LRP1 expression is essential for early embryonic development. LRP1 facilitates various biological processes including lipoprotein metabolism, proteinase regulation, lysosomal enzymes activation and entry of viruses and toxins into the cells. In addition, It also regulates cell signaling pathways, cell migration, and neurodevelopment. LRP1 has an ability to bind at least 30 different ligands such as lipoproteins, proteinases, proteinase-inhibitor complexes, extra cellular matrix (ECM) proteins and various intracellular proteins. It also binds to bacterial toxins and viruses.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Estrogen-enhanced apical and basolateral secretion of apolipoprotein B-100 by polarized trophoblast-derived BeWo cells
Kamper M, et al.
Biochimie, 138(10), 116-123 (2017)
LRP1 Suppresses Bone Resorption in Mice by Inhibiting the RANKL-Stimulated NF-$\kappa$B and p38 Pathways During Osteoclastogenesis
Lu Di
Journal of Bone and Mineral Research, 33(10), 1773-1784 (2018)
Beyond endocytosis: LRP function in cell migration, proliferation and vascular permeability
Lillis AP, et al.
Journal of Thrombosis and Haemostasis, 3(8), 1884-1893 (2005)
Inflammatory mediators promote production of shed LRP1/CD91, which regulates cell signaling and cytokine expression by macrophages
Gorovoy M, et al.
Journal of Leukocyte Biology, 88(4), 769-778 (2010)
Coralie Brifault et al.
The Journal of biological chemistry, 292(45), 18699-18712 (2017-10-04)
In the CNS, microglia are activated in response to injury or infection and in neurodegenerative diseases. The endocytic and cell signaling receptor, LDL receptor-related protein-1 (LRP1), is reported to suppress innate immunity in macrophages and oppose microglial activation. The goal

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