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ABN1475

Sigma-Aldrich

Anti-Serotonin receptor 3A/HTR3A

from rabbit, purified by affinity chromatography

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Synonym(s):
5-hydroxytryptamine receptor 3A, 5-HT-3, 5-HT3-A, 5-HT3A, 5-HT3R, 5-hydroxytryptamine receptor 3, Serotonin receptor 3A, Serotonin-gated ion channel receptor
eCl@ss:
32160702

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... HTR3A(3359)

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This Item
SAB4501483SAB4501469MAB11041
Anti-Serotonin receptor 3A/HTR3A from rabbit, purified by affinity chromatography

ABN1475

Anti-Serotonin receptor 3A/HTR3A

Quality Level

100

Quality Level

-

Quality Level

-

Quality Level

100

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

ELISA: 1:10000, immunofluorescence: 1:100-1:500, western blot: 1:500-1:1000

technique(s)

ELISA: 1:20000, western blot: 1:500-1:1000

technique(s)

immunohistochemistry: suitable, western blot: suitable

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

19A9.2, monoclonal

Gene Information

human ... HTR3A(3359)

Gene Information

human ... HTR5A(3361)

Gene Information

human ... HTR1A(3350)

Gene Information

human ... HTR1A(3350)
rat ... Htr1A(24473)

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General description

5-hydroxytryptamine receptor 3A (UniProt P46098; also known as 5-HT-3, 5-HT3-A, 5-HT3A, 5-HT3R, 5-hydroxytryptamine receptor 3, Serotonin receptor 3A, Serotonin-gated ion channel receptor) is encoded by the HTR3A (also known as 5HT3R, HTR3) gene (Gene ID 3359) in human. Hydroxytryptamine (5-HT) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT. 5-hydroxytryptamine receptor 3 (5-HT3R) is a ligand-gated ion channel involved in many neurophysiological functions and is widely expressed throughout the brain and in peripheral organs. Five different subunits (A through E) have been identified, with the homopentameric 5-HT3A being the most studied form. 5-HT3A is produced with a signal peptide sequence (a.a. 1-23), which is removed posttranslationally to yield the mature 4-transmembrane (a.a. 242-268, 274-292, 303-321, 456-475) ion channel with both its N- and C-terminal ends exposed extracellularly (a.a. 24-241 and 476-478).

Specificity

This rabbit polyclonal antibody detects human Serotonin receptor 3A/HTR3A by targeting a sequence within the second cytoplasmic domain.

Immunogen

KLH-conjugated linear peptide corresponding to a sequence from the second cytomplasmic domain of human Serotonin receptor 3A/HTR3A.

Application

Anti-Serotonin receptor 3A/HTR3A Antibody, Cat. No. ABN1475, is a highly specific rabbit polyclonal antibody that targets HTR3A and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected serotonin receptor 3A/HTR3A in human prostate cancer, as well as in normal cerebral cortex, heart and tonsil tissue sections.
Research Category
Neuroscience

Quality

Evaluated by Western Blotting in human cerebral cortex tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected serotonin receptor 3A/HTR3A in 10 µg of human cerebral cortex tissue lysate.

Target description

~55 kDa observed. 52,79/55.28 kDa (mature/pro-isoform 1; 5-HT3R-AS), 55.90/58.39 kDa (mature/pro-isoform 2; 5-HT3R-AL), 52.79/53.76 kDa (mature/pro-isoform 3), 52.79/55.89 kDa (mature/pro-isoform 4), 55.90/59.00 kDa (mature/pro-isoform 5). Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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