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Key Documents

ABN1658

Sigma-Aldrich

Anti-pro-TRH

serum, from rabbit

Synonym(s):

anti-thyrotropin-releasing hormone, anti-Ps4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat

species reactivity (predicted by homology)

mouse

technique(s)

immunofluorescence: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

rat ... Trh(25569)

General description

Pro-thyrotropin-releasing hormone (UniProt: P01150; also known as Pro-TRH, Prothyroliberin, TRH, TSH-releasing factor, Thyrotropin-releasing factor, TRF) is encoded by the Trh gene (Gene ID: 25569) in rat. TRH is a secreted hypothalamic protein that travels across the median eminence to the pituitary via the hypophyseal portal system. It functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems. Pro-TRH (TRH-potentiating peptide: Ps4) is co-synthesized with TRH serves as a marker for TRH-containing neurons. Like TRH itself, pro-TRH is biologically active.

Specificity

This polyclonal antibody detects Thyrotropin-releasing hormone-containing neurons in Rat Hypothalamus and spinal cord.

Immunogen

A linear peptide corresponding to 10 amino acids

Application

Anti-pro-TRH, Cat. No. ABN1658, is a highly specific rabbit polyclonal antibody, that targets pro- Thyrotropin-releasing hormone and has been tested in Immunofluorescence and Western Blotting.
Immunofluorescence Analysis: A representative lot detected pro-TRH in median eminence of rat and L2 spinal cord tissues. (Courtesy of Dr. Martin Wessendorf at University of Minnesota).

Immunofluorescence Analysis: A representative lot detected pro-TRH in rat brain stem & rat spinal cord tissues. (Wessendorf, M.W., et. al. (1990). J Histochem Cyotchem. 38(12):1859-77).
Research Category
Neuroscience

Quality

Evaluated by Western Blotting in rat hypothalamus tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected pro-TRH in 10 µg of rat hypothalamus tissue lysate.

Target description

~29 kDa observed; 29.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Rabbit polyclonal serum with 0.09% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1


Certificates of Analysis (COA)

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Ian E Gonzalez et al.
Endocrinology, 162(6) (2021-04-10)
The paraventricular nucleus of the hypothalamus (PVH) is a heterogeneous collection of neurons that play important roles in modulating feeding and energy expenditure. Abnormal development or ablation of the PVH results in hyperphagic obesity and defects in energy expenditure whereas

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