MilliporeSigma
All Photos(1)

Documents

ECM580

Sigma-Aldrich

QCM Haptotaxis Cell Migration Assay -Fibronectin, 24-well, colorimetric

The QCM Haptotaxis Cell Migration Assay, 24-well plate -Fibronectin, Colorimetric is ideal for the study of epithelial & fibroblast cell haptotaxis.

Sign Into View Organizational & Contract Pricing

eCl@ss:
32161000
NACRES:
NA.84

Quality Level

species reactivity

vertebrates

manufacturer/tradename

Chemicon®
QCM

technique(s)

cell based assay: suitable

detection method

colorimetric

shipped in

wet ice

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
ECM582ECM506ECM510
species reactivity

vertebrates

species reactivity

vertebrates

species reactivity

-

species reactivity

-

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®, QCM

manufacturer/tradename

Chemicon®, QCM

manufacturer/tradename

Chemicon®, QCM

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

fluorometric

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

Also available: Cell Comb Scratch Assay! Get biochemical data from a scratch assay!Click Here

Introduction
The CHEMICON QCM Haptotaxis Cell Migration Assay - Fibronectin, Colorimetric is ideal for the study of epithelial and fibroblast cell haptotaxis. The quantitative nature of this assay is especially useful for large scale screening of pharmacologic agents. BSA-coated control chambers provide an appropriate migration control. The 8 μm pore size of this assay′s Boyden chambers is not appropriate for the study of lymphocyte migration.
• Each kit provides sufficient materials for the evaluation of 12 samples.
• Please refer to the product insert for assay details.

The CHEMICON QCM Haptotaxis Cell Migration Assay - Fibronectin, Colorimetric assay is intended for research use only; not for diagnostic applications.

Application

Research Category
Cell Structure

Packaging

Sufficient for analysis of 12 samples

Components

Fibronectin Test Plate: (Part No. 2005689) One 24-well culture plate, containing 12 human FN-coated Boyden chambers, sufficient for the evaluation of 12 test samples.

BSA Control Plate: (Part No. 2005791) One 24-well culture plate, containing 12 BSA-coated Boyden chambers, sufficient for the evaluation of 12 controls.

Cell Stain Solution*: (Part No. 20294) One vial - 10 mL.

Extraction Buffer: (Part No. 20295) One vial - 10 mL.

24 well Stain Extraction Plate: (Part No. 2005871). One plate.

96 well Stain Quantitation Plate: (Part No. 2005870). One plate.

Swabs: (Part No. 10202) 50 each.

Forceps: (Part No. 10203) 1 pair

*Cell Stain Solution contains a small amount of crystal violet, which is toxic if swallowed or inhaled, and may cause irritation to the eyes, respiratory system, and skin. Handle with caution.

Linkage

Replaces: ECM500

Storage and Stability

Store at 4°C for up to one year after date of purchase.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Flame

signalword

Danger

Storage Class

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Liangping Yuan et al.
Molecular cancer, 12, 18-18 (2013-03-19)
Metastasis in medulloblastoma (MB) is associated with poor survival. Recent genetic studies revealed MB to comprise distinct molecular subgroups, including the sonic hedgehog (SHH) subgroup that exhibits a relatively high rate of progression. To identify targeted therapeutics against metastasis, a
Thamara J Abouantoun et al.
Molecular cancer therapeutics, 8(5), 1137-1147 (2009-05-07)
Platelet-derived growth factor (PDGF) receptor (PDGFR) expression correlates with metastatic medulloblastoma. PDGF stimulation of medulloblastoma cells phosphorylates extracellular signal-regulated kinase (ERK) and promotes migration. We sought to determine whether blocking PDGFR activity effectively inhibits signaling required for medulloblastoma cell migration
Astrid Hensel et al.
iScience, 25(6), 104355-104355 (2022-05-24)
The unique threonine protease Tasp1 impacts not only ordered development and cell proliferation but also pathologies. However, its substrates and the underlying molecular mechanisms remain poorly understood. We demonstrate that the unconventional Myo1f is a Tasp1 substrate and unravel the

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service