MilliporeSigma
All Photos(3)

Documents

ABN240

Sigma-Aldrich

Anti-Amyloid, β 1-40, aβ Antibody

from rabbit, purified by affinity chromatography

Sign Into View Organizational & Contract Pricing

Synonym(s):
Amyloid beta A4 protein, ABPP, APPI, APP, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II, 1.N-APP2.Soluble APP-alpha, S-APP-alpha3.Soluble APP-beta, S-APP-beta4.C995.Beta-amyloid protein 42, Be
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

bovine (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology)

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... APP(351)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
MABN639MABN2295MABN687
biological source

rabbit

biological source

mouse

biological source

rat

biological source

camel

antibody form

affinity isolated antibody

antibody form

affinity purified immunoglobulin

antibody form

purified antibody

antibody form

purified immunoglobulin

shipped in

wet ice

shipped in

wet ice

shipped in

ambient

shipped in

dry ice

clone

polyclonal

clone

1E8, monoclonal

clone

2E9, monoclonal

clone

B10, monoclonal

technique(s)

ELISA: suitable, western blot: suitable, immunohistochemistry: suitable (paraffin)

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ELISA: suitable, dot blot: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Amyloid β 1-40 is one of several functional peptides (39 to 43 amino acids in length) formed from proteolytic cleavage of the amyloid-beta precursor protein (APP) by the family of secretase enzymes. Although the APP protein is required for a number of biological functions including axonal transport, cell adhesion, and transcription, the amyloid β 1-40 peptide has been studied mostly for its role in neurotoxicity, particularly in the context of Alzheimer’s disease. Previous studies have reported that expression of amyloid β 1-40 increases in Alzheimer’s disease and correlate with the occurrence of aberrant synaptic structure and cognitive dysfunction. These detrimental effects on neural function may be related to aggregation of this peptide (formation of neurite plaques), as neurite plaques been shown to disrupt multiple neurotransmitter systems, and synaptic events such as long-term potentiation.

Specificity

This antibody recognizes the P3(40) peptide of Amyloid, β 1-40.

Immunogen

Epitope: P3(40) peptide
KLH-conjugated linear peptide corresponding to the P3(40) peptide of human Amyloid, B 1-40.

Application

Anti-Amyloid, β 1-40, aβ Antibody is an antibody against Amyloid, β 1-40, aβ for use in Western Blotting, IHC(P), ELISA.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Amyloid, B 1-40 in human Alzheimer′s diseased brain tissue.

ELISA: A representative lot detected Amyloid, B 1-40 in ELISA (Direct).
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Quality

Evaluated by Western Blot in human Alzheimer brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Amyloid, B 1-40 on 10 µg of human Alzheimer brain tissue lysate.

Target description

~4 kDa observed. An uncharacterized band appears at ~19 kDa in some lysates.

Linkage

Replaces: AB5737

Physical form

Affinity purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human Alzhiemer brain tissue lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Amanda B Chai et al.
International journal of molecular sciences, 22(1) (2021-01-02)
Defective clearance mechanisms lead to the accumulation of amyloid-beta (Aβ) peptides in the Alzheimer's brain. Though predominantly generated in neurons, little is known about how these hydrophobic, aggregation-prone, and tightly membrane-associated peptides exit into the extracellular space where they deposit
Xike Qin et al.
The Journal of biological chemistry, 291(42), 22276-22287 (2016-09-01)
Accumulation of amyloid-β peptide (Aβ) in the brain is regarded as central to Alzheimer's disease (AD) pathogenesis. Aβ is generated by a sequential cleavage of amyloid precursor protein (APP) by β-secretase 1 (BACE-1) followed by γ-secretase. BACE-1 cleavage of APP

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service