Skip to Content
MilliporeSigma
All Photos(3)

Key Documents

ABN477

Sigma-Aldrich

Anti-OTUD4 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

OTU domain-containing protein 4, HIV-1-induced protein HIN-1, OTUD4

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

UniProt accession no.

Q01804

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... OTUD4(54726)

General description

OTU domain-containing protein 4 (OTUD4) is a member of the OTU (ovarian tumor) domain containing cysteine protease superfamily, in which the OTU domain generally confers deubiquitinase activity. High levels of OTUD4 are found in lung, ovary, testis, liver, heart skeletal muscle, kidney, and brain, with lower levels present in pancreas and spleen. Four isoforms of OTUD4 are known to exist, the shortest one being expressed specifically in HIV-1-infected cells.

Immunogen

KLH-conjugated linear peptide corresponding to human OTUD4.

Application

Detect OTU domain-containing protein 4 using this rabbit polyclonal antibody, Anti-OTUD4 Antibody validated for use in western blotting, ICC & Immunofluorescence.
Immunocytochemistry Analysis: 2.5 µg/mL from a representative lot detected OTUD4 in Daudi cells.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected OTUD4 in Daudi cells.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blotting in Daudi cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected OTUD4 in 15 µg of Daudi cell lysate.

Target description

~108 kDa observed. Uniprot describes four isoforms at ~17 kDa, ~24 kDa, ~117 kDa, and ~124 kDa Uncharacterized bands may appear in some lysates.

Physical form

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Daudi cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Zhiqiang Wu et al.
Cancer cell international, 19, 99-99 (2019-04-24)
Radiotherapy is becoming one major therapeutics for non-small cell lung cancer (NSCLC). Identifying novel radiosensitizers will greatly increase the efficacy of radiotherapy and benefit more patients. OTU deubiquitinase 4 (OTUD4) has been reported involved in DNA damage repair pathways and

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service