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A9045

Sigma-Aldrich

Agarose, low gelling temperature

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture

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Synonym(s):
2-Hydroxyethyl agarose
CAS Number:
39346-81-1
MDL number:
MFCD00081294
NACRES:
NA.21

product line

BioReagent

Quality Level

200

form

powder

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

EEO

≤0.1

mp

≤65 °C ( at a 1.5% gel)

transition temp

congealing temperature 26-30 °C

gel strength

≥200 g/cm2 (1% gel)

solubility

H2O: 10 mg/mL (with heat)

anion traces

sulfate (SO42-): ≤0.10%

foreign activity

RNase and DNase free

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Agarose, low gelling temperature BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture

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Agarose, low gelling temperature

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Agarose, low gelling temperature

Agarose, low gelling temperature BioReagent, for molecular biology

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Agarose, low gelling temperature

form

powder

form

powder

form

powder

form

powder

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable

technique(s)

electrophoresis: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

EEO

≤0.1

EEO

≤0.12

EEO

≤0.10

EEO

≤0.10

transition temp

congealing temperature 26-30 °C

transition temp

gel point 26 °C ±2 °C (1.5% gel)

transition temp

congealing temperature 26-30 °C

transition temp

congealing temperature 26-30 °C

gel strength

≥200 g/cm2 (1% gel)

gel strength

≥250 g/cm2 (1% gel)

gel strength

≥200 g/cm2 (1% gel)

gel strength

≥200 g/cm2 (1% gel)

General description

Agarose is an algal polysaccharide. Agarose is a thermoreversible, ion-dependent gelling agent. Agarose gel electrophoresis is useful for the clinical routine analyses of proteins in plasma and other body fluids. It is a low gelling temperature derivative with unique gelling properties. Gels forms at <30°C, remelt at temperatures in excess of 60°C. Gels exhibit excellent clarity and are particularly useful for the preparation of media containing heat-labile materials.

Application

Agarose, a low gelling temperature derivative, is the suitable reagent for:

  • plant cell culture studies
  • insect cell culture studies
  • cell culture studies

It may be used for the following studies:
  • Recovery of defined RNA and DNA fractions after electrophoretic separation.
  • Cytochemical staining procedure to investigate the succinate dehydrogenase (SDH) activity in pre-ovulatory mouse oocytes.
  • Purification of RNA in Caenorhabditis elegans by electrophoresis.

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Patrick M Ferree et al.
Scientific reports, 9(1), 12194-12194 (2019-08-23)
Males of hymenopteran insects, which include ants, bees and wasps, develop as haploids from unfertilized eggs. In order to accommodate their lack of homologous chromosome pairs, some hymenopterans such as the honeybee have been shown to produce haploid sperm through
Nariko Arimura et al.
Science advances, 6(36) (2020-09-13)
For normal neurogenesis and circuit formation, delamination of differentiating neurons from the proliferative zone must be precisely controlled; however, the regulatory mechanisms underlying cell attachment are poorly understood. Here, we show that Down syndrome cell adhesion molecule (DSCAM) controls neuronal
Agarose gel electrophoresis.
Johansson B G.
Scandinavian Journal of Clinical and Laboratory Investigation, 29(S124), 7-19 (1972)
A Fire et al.
Nature, 391(6669), 806-811 (1998-03-05)
Experimental introduction of RNA into cells can be used in certain biological systems to interfere with the function of an endogenous gene. Such effects have been proposed to result from a simple antisense mechanism that depends on hybridization between the
A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels.
L Wieslander
Analytical biochemistry, 98(2), 305-309 (1979-10-01)
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