A9045
Agarose, low gelling temperature
BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture
Synonym(s):
2-Hydroxyethyl agarose
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About This Item
Recommended Products
product line
BioReagent
Quality Level
form
powder
technique(s)
cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable
EEO
≤0.1
mp
≤65 °C ( at a 1.5% gel)
transition temp
congealing temperature 26-30 °C
gel strength
≥200 g/cm2 (1% gel)
solubility
H2O: 10 mg/mL (with heat)
anion traces
sulfate (SO42-): ≤0.10%
1 of 4
This Item | A5030 | A2576 | A2929 |
---|---|---|---|
form powder | form powder | form powder | form powder |
EEO ≤0.14 | EEO ≤0.05 | EEO ≤0.12 | EEO ≤0.10 |
Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
impurities ≤10% water | impurities - | impurities ≤7% water | impurities ≤10% water |
anion traces sulfate (SO42-): ≤0.20% | anion traces - | anion traces sulfate (SO42-): ≤0.14% | anion traces sulfate (SO42-): ≤0.20% |
General description
Application
- plant cell culture studies
- insect cell culture studies
- cell culture studies
It may be used for the following studies:
Analysis Note
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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