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Recommended Products
type
Type VII-A
Quality Level
form
powder
technique(s)
electrophoresis: suitable
impurities
≤7% water
ash
≤0.4%
turbidity
≤4 NTU (1.5% gel)
EEO
≤0.12
mp
65 °C±1 °C
transition temp
gel point 26 °C ±2 °C (1.5% gel)
gel strength
≥250 g/cm2 (1% gel)
anion traces
sulfate (SO42-): ≤0.4%
Related Categories
1 of 4
This Item | A4018 | A9045 | A9414 |
|---|---|---|---|
| technique(s) electrophoresis: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable | technique(s) - |
| impurities ≤7% water | impurities - | impurities - | impurities - |
| EEO ≤0.12 | EEO ≤0.10 | EEO ≤0.1 | EEO ≤0.10 |
| transition temp gel point 26 °C ±2 °C (1.5% gel) | transition temp congealing temperature 26-30 °C | transition temp congealing temperature 26-30 °C | transition temp congealing temperature 26-30 °C |
| gel strength ≥250 g/cm2 (1% gel) | gel strength ≥200 g/cm2 (1% gel) | gel strength ≥200 g/cm2 (1% gel) | gel strength ≥200 g/cm2 (1% gel) |
General description
Application
Agarose has been used:
- to encapsulate Escherichia coli on a hydrogel in tissue culture
- to entrap Aliivibrio fischeri on a disposable card involved in designing of toxicity biosensors
- as a the dispersed phase of emulsion during preparation agar beads
Biochem/physiol Actions
Analysis Note
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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