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Key Documents

A7431

Sigma-Aldrich

Agarose

BioReagent, for molecular biology, Wide range/Standard 3:1

Synonym(s):

3,6-Anhydro-α-L-galacto-β-D-galactan

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41105317
PubChem Substance ID:
NACRES:
NA.25

biological source

algae (marine)

Quality Level

grade

for molecular biology

product line

BioReagent

form

powder

technique(s)

electrophoresis: suitable

EEO

≤0.13

mp

≤90 °C

transition temp

gel point 32.5-38 °C (4% gel)

gel strength

≥1200 g/cm2 (4% gel)

anion traces

sulfate (SO42-): ≤0.15%

foreign activity

DNase, RNase, NICKase, none detected

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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Application

Specially formulated to form strong, flexible gels for separation of small (≤1 kb) PCR products, DNA and RNA. Stronger gels make gel-handling easier. Sharp resolution is produced with DNA fragments ranging from 10-1500 bp.

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Tobias K H Müller et al.
Journal of chromatography. A, 1285, 97-109 (2013-03-14)
An integrated approach to temperature-controlled chromatography, involving copolymer modified agarose adsorbents and a novel travelling cooling zone reactor (TCZR) arrangement, is described. Sepharose CL6B was transformed into a thermoresponsive cation exchange adsorbent (thermoCEX) in four synthetic steps: (i) epichlorohydrin activation;
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Elevated triglycerides are associated with an increased risk of cardiovascular disease, and lipoprotein lipase (LPL) is the rate-limiting enzyme for the hydrolysis of triglycerides from circulating lipoproteins. The N-terminal domain of angiopoietin-like protein 4 (ANGPTL4) inhibits LPL activity. ANGPTL4 was

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