MilliporeSigma
All Photos(3)

Documents

05066

Sigma-Aldrich

Agarose

High EEO, for molecular biology

Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose HE
CAS Number:
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

form

powder

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

ign. residue

≤1%

loss

≤10% loss on drying

EEO

0.23-0.27

transition temp

gel point 34-37 °C (1.5% solution)

gel strength

≥1500 g/cm2

anion traces

chloride (Cl-): ≤0.3%
sulfate (SO42-): ≤0.6%

cation traces

Ca: ≤0.05%
Cd: ≤0.001%
Co: ≤0.001%
Cr: ≤0.001%
Cu: ≤0.001%
Fe: ≤0.001%
K: ≤0.05%
Mg: ≤0.001%
Mn: ≤0.001%
Na: ≤0.5%
Ni: ≤0.001%
Pb: ≤0.001%
Zn: ≤0.001%

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
A5030A7174A4679
Agarose High EEO, for molecular biology

Sigma-Aldrich

05066

Agarose

Agarose Ultra-low Gelling Temperature

Sigma-Aldrich

A5030

Agarose

Agarose High Gelling Temperature

Sigma-Aldrich

A7174

Agarose

Agarose Low EEO, for Immunoelectrophoresis

Sigma-Aldrich

A4679

Agarose

form

powder

form

powder

form

powder

form

powder

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

impurities

-

impurities

≤10% water

impurities

≤7% water

EEO

0.23-0.27

EEO

≤0.05

EEO

≤0.14

EEO

0.09-0.13

transition temp

gel point 34-37 °C (1.5% solution)

transition temp

gel point 8-17 °C

transition temp

gel point 41 °C ±1.5 °C (1.5% gel)

transition temp

gel point 34-38 °C (1.5% gel)

gel strength

≥1500 g/cm2

gel strength

-

gel strength

≥900 g/cm2 (1% gel)

gel strength

≥1200 g/cm2 (1% gel)

Application

Agarose is used for preparative and analytical separation of nucleic acids. It can be used for single cell gel electrophoresis assay. It can also be used for southern blotting. It can also be used for assessing transepithelial ionic fluxes from cultured neonatal rat semicircular canal epithelium.

Biochem/physiol Actions

Agarose is a polysaccharide used for resolving DNA and RNA fragments from 500 - 20,000 bp. It provides strong gel structure which assists in better handling and less breakage. It can also reexpress the differentiated collagen phenotype from dedifferentiated chondrocytes during agarose gel culture.

Packaging

Packaging
50, 500 g in poly bottle

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 6

1 of 6

Agarose Type IV, Special High EEO

Sigma-Aldrich

A3643

Agarose

Agarose Type I-A, low EEO

Sigma-Aldrich

A0169

Agarose

Agarose Low EEO, for Immunoelectrophoresis

Sigma-Aldrich

A4679

Agarose

Agarose For pulsed field electrophoresis running gel

Sigma-Aldrich

A2929

Agarose

Agarose Ultra-low Gelling Temperature, molecular biology grade

Sigma-Aldrich

A2576

Agarose

C M Hughes et al.
Molecular human reproduction, 2(8), 613-619 (1996-08-01)
Baseline DNA damage in spermatozoa from fertile and infertile men was compared using a modified alkali single cell gel electrophoresis (comet) assay. Semen from normozoospermic fertile, normozoospermic infertile and asthenozoospermic infertile (World Health Organization criteria, 1992) samples were studied. No
K C Reed et al.
Nucleic acids research, 13(20), 7207-7221 (1985-10-25)
The unique properties of nylon membranes allow for dramatic improvement in the capillary transfer of DNA restriction fragments from agarose gels (Southern blotting). By using 0.4 M NaOH as the transfer solvent following a short pre-treatment of the gel in
P D Benya et al.
Cell, 30(1), 215-224 (1982-08-01)
The differentiated phenotype of rabbit articular chondrocytes consists primarily of type II collagen and cartilage-specific proteoglycan. During serial monolayer culture this phenotype is lost and replaced by a complex collagen phenotype consisting predominately of type I collagen and a low
Pierre G Milhaud et al.
American journal of physiology. Cell physiology, 283(6), C1752-C1760 (2002-10-22)
The ductal epithelium of the semicircular canal forms much of the boundary between the K+-rich luminal fluid and the Na+-rich abluminal fluid. We sought to determine whether the net ion flux producing the apical-to-basal short-circuit current (I(sc)) in primary cultures
Aurica Precupas et al.
Chemical research in toxicology, 33(8), 2054-2071 (2020-07-01)
Understanding nanomaterial (NM)-protein interactions is a key issue in defining the bioreactivity of NMs with great impact for nanosafety. In the present work, the complex phenomena occurring at the bio/nano interface were evaluated in a simple case study focusing on

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service