A2929
Agarose
For pulsed field electrophoresis running gel
Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan
About This Item
Recommended Products
biological source
algae (red)
Quality Level
form
powder
technique(s)
electrophoresis: suitable
impurities
≤10% water
EEO
≤0.10
transition temp
gel point 38-43 °C (10% gel)
gel strength
≥2000 g/cm2
anion traces
sulfate (SO42-): ≤0.20%
foreign activity
DNase, RNase, NICKase, none detected
InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
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Related Categories
Specificity
Application
Features and Benefits
- Low EEO allows for increased voltages and more rapid DNA migration
- Greater gel strength allows for stability of lower concentration gels leading to faster DNA migration
- Gels exhibit low background fluorescence using ethidium bromide staining
Analysis Note
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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