Skip to Content
MilliporeSigma
All Photos(4)

Documents

A2929

Sigma-Aldrich

Agarose

For pulsed field electrophoresis running gel

Synonym(s):

3,6-Anhydro-α-L-galacto-β-D-galactan

Sign Into View Organizational & Contract Pricing


About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41105317
PubChem Substance ID:
NACRES:
NA.25

biological source

algae (red)

Quality Level

form

powder

technique(s)

electrophoresis: suitable

impurities

≤10% water

EEO

≤0.10

transition temp

gel point 38-43 °C (10% gel)

gel strength

≥2000 g/cm2

anion traces

sulfate (SO42-): ≤0.20%

foreign activity

DNase, RNase, NICKase, none detected

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

Looking for similar products? Visit Product Comparison Guide

Specificity

Suitable for the separation of high molecular weight DNA. Gels are easy to handle and give faster separation and better resolution of high molecular weight DNA by field inversion electrophoresis than our routine-use agarose.

Application

Especially formulated for fast resolution of large DNA (10-2000 ·kb) by pulsed-field gel electrophoresis (PFGE) or conventional electrophoresis. Pulsed-field gel electrophoresis was developed in 1984 to deal with "reptation" of large DNA′s whose size surpass the limit of resolution and all migrate at the same rate. Alternately pulsed, orthogonally oriented electric fields are applied across the gel. Every time the direction of the electric field changes, the large DNA′s can no longer migrate until they become reoriented along the new electric field. The time required for reorientation is dependent on the size of the DNA, therefore the DNA is fractionated by size.

Features and Benefits

  • Low EEO allows for increased voltages and more rapid DNA migration
  • Greater gel strength allows for stability of lower concentration gels leading to faster DNA migration
  • Gels exhibit low background fluorescence using ethidium bromide staining

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 7

1 of 7

Millipore

Millipore

1.01236

Agarose

Agarose Medium EEO, for molecular biology

Sigma-Aldrich

A6877

Agarose

Agarose Special High EEO

Sigma-Aldrich

A5304

Agarose

Agarose Type I-A, low EEO

Sigma-Aldrich

A0169

Agarose

Agarose Ultra-low Gelling Temperature, molecular biology grade

Sigma-Aldrich

A2576

Agarose

Agarose Type IV, Special High EEO

Sigma-Aldrich

A3643

Agarose

Sifang Liao et al.
Frontiers in endocrinology, 11, 180-180 (2020-05-07)
The insulin/IGF-signaling pathway is central in control of nutrient-dependent growth during development, and in adult physiology and longevity. Eight insulin-like peptides (DILP1-8) have been identified in Drosophila, and several of these are known to regulate growth, metabolism, reproduction, stress responses
Yu-Hsuan Cheng et al.
BMC biology, 18(1), 180-180 (2020-12-01)
Ciliates are an ancient and diverse eukaryotic group found in various environments. A unique feature of ciliates is their nuclear dimorphism, by which two types of nuclei, the diploid germline micronucleus (MIC) and polyploidy somatic macronucleus (MAC), are present in
Michael J Lafferty et al.
The Journal of biological chemistry, 288(40), 28524-28534 (2013-08-21)
Elevated triglycerides are associated with an increased risk of cardiovascular disease, and lipoprotein lipase (LPL) is the rate-limiting enzyme for the hydrolysis of triglycerides from circulating lipoproteins. The N-terminal domain of angiopoietin-like protein 4 (ANGPTL4) inhibits LPL activity. ANGPTL4 was
Julia Dahlmann et al.
Biomaterials, 34(10), 2463-2471 (2013-01-22)
In most pluripotent stem cell differentiation protocols the formation of embryoid bodies (EBs) is an important step. Here we describe a rapid, straightforward soft lithography approach for the preparation of hydrophilic silicon masters from different templates and the subsequent production
Sujith V W Weerasinghe et al.
Journal of medicinal chemistry, 51(18), 5542-5551 (2008-08-30)
Histone deacetylase 1 (HDAC1) has been linked to cell growth and cell cycle regulation, which makes it a widely recognized target for anticancer drugs. Whereas variations of the metal-binding and capping groups of HDAC inhibitors have been studied extensively, the

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service