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A2929

Sigma-Aldrich

Agarose

For pulsed field electrophoresis running gel

Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan
CAS Number:
EC Number:
MDL number:
PubChem Substance ID:

biological source

algae (red)

Quality Level

form

powder

technique(s)

electrophoresis: suitable

impurities

≤10% water

EEO

≤0.10

transition temp

gel point 38-43 °C (10% gel)

gel strength

≥2000 g/cm2

anion traces

sulfate (SO42-): ≤0.20%

foreign activity

DNase, RNase, NICKase, none detected

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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This Item
A9539A5304A2790
Agarose For pulsed field electrophoresis running gel

Sigma-Aldrich

A2929

Agarose

Agarose BioReagent, for molecular biology, low EEO

Sigma-Aldrich

A9539

Agarose

Agarose Special High EEO

Sigma-Aldrich

A5304

Agarose

Agarose Wide range, for molecular biology

Sigma-Aldrich

A2790

Agarose

form

powder

form

powder

form

powder

form

powder

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable, immunoelectrophoresis: suitable

technique(s)

electrophoresis: suitable

impurities

≤10% water

impurities

≤10% moisture content

impurities

≤11% moisture content

impurities

-

EEO

≤0.10

EEO

0.09-0.13

EEO

≤0.40

EEO

≤0.15

transition temp

gel point 38-43 °C (10% gel)

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

transition temp

gel point ≤35 °C

Specificity

Suitable for the separation of high molecular weight DNA. Gels are easy to handle and give faster separation and better resolution of high molecular weight DNA by field inversion electrophoresis than our routine-use agarose.

Application

Especially formulated for fast resolution of large DNA (10-2000 ·kb) by pulsed-field gel electrophoresis (PFGE) or conventional electrophoresis. Pulsed-field gel electrophoresis was developed in 1984 to deal with "reptation" of large DNA′s whose size surpass the limit of resolution and all migrate at the same rate. Alternately pulsed, orthogonally oriented electric fields are applied across the gel. Every time the direction of the electric field changes, the large DNA′s can no longer migrate until they become reoriented along the new electric field. The time required for reorientation is dependent on the size of the DNA, therefore the DNA is fractionated by size.

Features and Benefits

  • Low EEO allows for increased voltages and more rapid DNA migration
  • Greater gel strength allows for stability of lower concentration gels leading to faster DNA migration
  • Gels exhibit low background fluorescence using ethidium bromide staining

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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