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AP181P

Sigma-Aldrich

Goat Anti-Mouse IgG Antibody, HRP conjugate, Species Adsorbed

0.8 mg/mL, Chemicon®

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eCl@ss:
32160702
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

concentration

0.8 mg/mL

technique(s)

ELISA: suitable
western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

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This Item
AP124AAP180PAP182P
biological source

goat

biological source

goat

biological source

donkey

biological source

donkey

species reactivity

mouse

species reactivity

mouse

species reactivity

goat

species reactivity

rabbit

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, western blot: suitable

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Application

EIA and Western blots: 1:5,000-1:100,000.

Immunohistochemistry: 1:500-1:5,000.

Optimal working dilutions must be determined by end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
This Goat anti-Mouse IgG Antibody, HRP conjugate, Species Adsorbed is validated for use in ELISA, WB for the detection of Mouse IgG.

Physical form

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25M NaCl, pH 7.6 with 15 mg/mL BSA.

RECONSTITUTION:

Reconstitute with 500 μL of sterile distilled water.

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

WARNING:

Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Efficacy of epidermal growth factor receptor-targeted molecular therapy in anaplastic thyroid cancer cell lines.
Nobuhara, Y; Onoda, N; Yamashita, Y; Yamasaki, M; Ogisawa, K; Takashima, T; Ishikawa, T; Hirakawa, K
British Journal of Cancer null
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It is being increasingly appreciated that the immunomodulatory functions of PARP1 inhibitors (PARPi) underlie their clinical activities in various BRCA-mutated tumors. PARPi possess both PARP1 inhibition and PARP1 trapping activities. The relative contribution of these two mechanisms toward PARPi-induced innate

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