CC102A
Human Integrin αVβ3, Octyl-β-D-glucopyranoside Formulation
Synonym(s):
Vitronectin Receptor, CD51/CD61
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41
Recommended Products
biological source
human
Quality Level
assay
>95% (silver stain, SDS-PAGE)
form
liquid
manufacturer/tradename
Chemicon®
concentration
0.25 mg/mL
NCBI accession no.
UniProt accession no.
Gene Information
human ... ITGAV(3685)
General description
Integrin alphaVbeta3 was purified from human placenta by affinity chromatography using immobilized monoclonal antibodies to alphaVbeta3 integrin. A tissue detergent extract, which was applied to the column was prepared as previously described [Belkin, 1990]. Product was tested and found negative for HIV and Hepatitis B.
Application
Electrophoresis and Immunoblotting control. Also useful for in vitro binding experiments with metalloproteinase MMP-2 .
Physical form
Purified protein inTris buffered saline with 100 mM Octyl-beta-D-glucopyranoside, and sodium azide. This format of alphaVbeta3 is appropriate for functional evaluations.
Storage and Stability
Maintain at -70°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/ thaw cycles.
Analysis Note
Specific Activity: Confirmed by vWf (von Willibrand factor) binding assay. It is expected that this preparation of alphaVbeta3 integrin will also interact with other alphaVbeta3 binding proteins in an RGD-dependent fashion.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Integrin alphaVbeta3 Binds to the RGD motif of glycoprotein B of Kaposi's sarcoma-associated herpesvirus and functions as an RGD-dependent entry receptor.
H Jacques Garrigues,Yelena E Rubinchikova,C Michael Dipersio,Timothy M Rose
Journal of virology null
V M Belkin et al.
The Journal of cell biology, 111(5 Pt 1), 2159-2170 (1990-11-01)
A membrane glycoprotein complex was isolated and purified from human smooth muscle by detergent solubilization and affinity chromatography on collagen-Sepharose. The complex was identified as VLA-1 integrin and consisted of two subunits of 195 and 130 kD in SDS-PAGE. Liposomes
Regulation of trophoblast beta1-integrin expression by contact with endothelial cells
Thirkill, TL et al.
Cell communication and signaling : CCS, 2(1), 4-4 (2004)
V M Belkin et al.
Biochemistry and molecular biology international, 40(1), 53-60 (1996-09-01)
Integrins of the beta 1 family were isolated from human smooth muscle. SDS-PAGE analysis and subsequent immunoblotting demonstrated that integrin samples contain a protein immunologically related to beta 1 integrin subunit with the previously undescribed apparent molecular mass 205 kD.
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